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Characterization of Presynaptic Muscarinic Receptors in Central Cholinergic Neurons

  • J. C. Szerb
Part of the Advances in Behavioral Biology book series (ABBI, volume 24)

Abstract

It is now well established that anticholinergic agents such as atropine increase the evoked release of ACh from the cerebral cortex in vivo (15, 22) and from cortical slices in vitro (2, 3). At a previous symposium on cholinergic mechanisms (23), observations were summarized which indicated that this enhancement of ACh release by antimuscarinic agents is the result of their overcoming the inhibitory effect of ACh which accumulates in unphysiologically high concentrations when a ChE inhibitor is used to prevent the hydrolysis of ACh (23). Evidence for this was obtained by measuring the evoked release of labelled ACh without inhibiting ChE (18, 21). The rate of evoked release of the label was reduced when a ChE inhibitor was present and restored when atropine was also added (3, 25). Our recent experiments attempted to answer the following questions: 1) Does the inhibitory effect of a ChE inhibitor require the presence of cell bodies of cholinergic neurons on release, or is this an effect on the terminals? 2) Has this feedback inhibition a physiological role, i. e. does it occur in the absence of ChE inhibition? 3) What is the sensitivity of this feedback inhibition to muscarinic agonists and antagonists?

Keywords

Medial Septum Muscarinic Agonist Postsynaptic Receptor Cholinergic Mechanism Presynaptic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Aghajanian, J.K., Haigler, H.J. and Bloom, F.E. (1972): Life Sci. l1:615–622.CrossRefGoogle Scholar
  2. 2.
    Rertels-Meeuws, M. M. and Polak, R.L. (1968): Brit. J. Pharmacol. 33:368–380.Google Scholar
  3. 3.
    Bourdois, P.S., Mitchell, J. F., Somogyi, G.T. and Szerb, J.C. (1974): Brit. J. Pharmacol. 52:509–517.Google Scholar
  4. 4.
    Bunney, B. S. and Aghajanian, G. K. (1975): In: Pre- and Postsynaptic Receptors, (Eds.) E. Usdin and W. E. Bunny, Jr. Marcel Dekker, New York, pp. 89–122.Google Scholar
  5. 5.
    Grewaal, D.S. and Quastel, J.H. (1973): Biochem. J. 132:1–14.PubMedGoogle Scholar
  6. 6.
    Hadhazy, P. and Szerb, J. C. (1977): Brain Res. 123:311–322.PubMedCrossRefGoogle Scholar
  7. 7.
    Kato, A.C., Collier, B., Ilson, D. and Wright, J. M. (1975): Canad. J. Physiol. Pharmacol. 53:1050–1057.CrossRefGoogle Scholar
  8. 8.
    Krnjevic, K. and Silver, A. (1965): J. Anat. 99:711–759.PubMedGoogle Scholar
  9. 9.
    Kuhar, M. J., Sethy, V.H., Roth, R. H. and Aghajanian, G.K. (1973): J. Neurochem. 20:581–593.PubMedCrossRefGoogle Scholar
  10. 10.
    Lewis, P. R., Shute, C.C.D. and Silver, A. (1967): J. Physiol. (Lond.) 191:215–224.Google Scholar
  11. 11.
    McGeer, E.G., Fibiger, H. C., McGeer, P. L. and Brooke, S. (1973): Brain Res. 52:289–300.PubMedCrossRefGoogle Scholar
  12. 12.
    McGeer, E.G., Wada, J.A., Terao, A. and Jung, E. (1969): Exp. Neurol. 24:277–284.PubMedCrossRefGoogle Scholar
  13. 13.
    McGeer, P. L., McGeer, E.G., Fibiger, H. C. and Wickson, V. (1971): Brain Res. 35:308–314.PubMedCrossRefGoogle Scholar
  14. 14.
    McGeer, P. L., McGeer, E.G., Singh, V.K. and Chase, W.H. (1974): Brain Res. 35:308–314.CrossRefGoogle Scholar
  15. 15.
    Mitchell, J.F. (1963): J. Physiol. (Lond.) 165:98–118.Google Scholar
  16. 16.
    Molenaar, P.C., Polak, R. L. and Nickolson, V.J. (1973): J. Neurochem. 21:667–678.CrossRefGoogle Scholar
  17. 17.
    Racagni, G., Trabucchi, M. and Cheney, D. L. (1975): N.-S. Arch. Pharmacol. 290:99–105.CrossRefGoogle Scholar
  18. 18.
    Richardson, I.W. and Szerb, J. C. (1974): Brit. J. Pharmacol. 52:499–507.Google Scholar
  19. 19.
    Richter, J.A. (1976): J. Neurochem. 26:791–797.PubMedCrossRefGoogle Scholar
  20. 20.
    Rommelspacher, H. and Kuhar, M.J. (1975): N.-S. Arch. Pharmacol. 291:17–21.CrossRefGoogle Scholar
  21. 21.
    Somogyi, G.T. and Szerb, J. C. (1972): J. Neurochem. 19:2667–2677.PubMedCrossRefGoogle Scholar
  22. 22.
    Szerb, J. C. (1964): Canad. J. Physiol. Pharmacol. 42:303–314.CrossRefGoogle Scholar
  23. 23.
    Szerb, J.C. (1975): In: Cholinergic Mechanisms. (Ed.) P. G. Waser, Raven Press, New York, pp. 213–216.Google Scholar
  24. 24.
    Szerb, J. C., Hadhazy, P. and Dudar, J.D. (1977): Brain Res. (in press).Google Scholar
  25. 25.
    Szerb, J. C. and Somogyi, G.T. (1973): Nature, New Biol. 241:121–122.Google Scholar
  26. 26.
    Yamamura, H.I. and Synder, S.H. (1974): Brain Res. 78:320–326.PubMedCrossRefGoogle Scholar
  27. 27.
    Yamamura, H.I. and Snyder, S.H. (1974): Proc. Nat. Acad. Sci. (Wash.) 71:1725–1729.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. C. Szerb
    • 1
  1. 1.Department of Physiology and BiophysicsDalhousie UniversityHalifaxCanada

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