Uptake of Choline in Nerve Cell Cultures: Correlation with the Endogenous Pool of Choline

  • R. Massarelli
Part of the Advances in Behavioral Biology book series (ABBI, volume 24)


The uptake of choline (Ch) in primary (20) or clonal (9, 20) nerve cell cultures presents different kinetic parameters which have been suggested to belong to two distinct mechanisms of uptake. Much information has recently appeared in the literature on the influence of one of these parameters, the high affinity (HA) uptake of Ch on the regulation of ACh metabolism in synaptosomes (2, 8, 11, 33). Earlier works (7, 14, 32) based on partially purified choline acetyltransferase (CAT: E. C. 2.3.1. 6) have in fact suggested that the rate limiting step in ACh turnover seems to be independent of the activity of CAT and rather dependent on the availability of the two substrates intervening in ACh synthesis: acetyleoenzyme A (AcCoA) and Ch. These compounds would then regulate ACh metabolism by mass action (7).


Nerve Cell Culture Wheat Germ Agglutinin Choline Uptake High Affinity Uptake Endogenous Pool 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • R. Massarelli
    • 1
  1. 1.Center of Neurochemistry, CNRSInstitute of Biological ChemistryStrasbourg CedexFrance

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