Regulation of Acetylcholine Synthesis in Rat Brain

  • R. L. Polak
  • P. C. Molenaar
  • P. Braggaar-Schaap
Part of the Advances in Behavioral Biology book series (ABBI, volume 24)


The question of how the level of ACh in the brain is controlled has not yet been settled. It has been suggested that it is controlled by mass action (22) or by product inhibition of the enzyme choline acetyltransferase (CAT: E. C. Another possibility is that ACh synthesis is controlled by the availability of the precursors choline (Ch) and/or acetyl-CoA (AcCoA) at the site of ACh synthesis. The present paper deals with evidence which could have a bearing on these possibilities. Two series of experiments are reviewed which were recently performed in our laboratories; 1) Ch uptake by cortical slices from rat brain (21) and 2) AcCoA synthesis by hyposmotically treated synaptosomal fractions.


Subcellular Fraction Brain Mitochondrion Cortical Slice High Affinity Uptake Acetylcholine Synthesis 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • R. L. Polak
    • 1
  • P. C. Molenaar
    • 2
  • P. Braggaar-Schaap
    • 2
  1. 1.Medical Biological Laboratory TNORijswijkZ.H.
  2. 2.Pharmacological Laboratory, Sylvius LaboratoriesUniversity of LeidenThe Netherlands

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