Control of Acetylcholine Synthesis in Motor Nerve Terminals

  • G. Pilar
  • R. Beach
  • K. Vaca
  • J. Suszkiw
Part of the Advances in Behavioral Biology book series (ABBI, volume 24)


This chapter is a summary of work published or in the final stages of publication done in this laboratory on the regulation of ACh synthesis in motor nerve terminals (2, 20, 21). Most of the present knowledge concerning transmitter release processes in nerve terminals is derived from electrophysiological investigations of the neuromuscular junction (11). ACh is secreted, probably by exocytosis from some protected compartment in the nerve terminals (most likely synaptic vesicles) (12, and Macintosh, this volume), in which it is stored after being locally synthesized. Choline (Ch), the substrate for ACh synthesis, is taken up from the plasma by a carrier-mediated mechanism (6). Previous investigations utilizing the rat diaphragm preparation showed that the small accumulation of Ch by nerve terminals could not be studied directly because more of the Ch was taken up by non-neural cells (3, 15). This is unfortunate because it has been shown that synaptosomes isolated from regions of the brain rich in cholinergic nerve terminals take up Ch via a sodium-dependent high affinity uptake system (SDHACU (1, 8, 10, 23). It would thus be highly advantageous to be able to study this process in a peripheral system in which physiological experiments could be carried out.


Nerve Terminal Neuromuscular Junction Cholinergic Neuron Motor Nerve Terminal Acetylcholine Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Barker, L.A. and Mittag, T.W. (1975): J. Pharmacol. Exp. Ther. 192:86–94.Google Scholar
  2. 2.
    Beach, R., Vaca, K. and Pilar, G. (1977): (in preparation).Google Scholar
  3. 3.
    Chang, C. C. and Lee, C. (1970): Neuropharmacology 9:223–233.PubMedCrossRefGoogle Scholar
  4. 4.
    Chiappinelli, V., Giacobini, E., Pilar, G. and Uchimura, H. (1976): J. Physiol. (Lond.) 257:749–766.Google Scholar
  5. 5.
    Fonnum, F. (1970): J. Neurochem. 17:1029–1037.PubMedCrossRefGoogle Scholar
  6. 6.
    Fonnum, F. (1973): Brain Bes. 62:497–507.CrossRefGoogle Scholar
  7. 7.
    Glover, V.A.S. and Potter, L.T. (1969): J. Neurochem. 18:571–589.CrossRefGoogle Scholar
  8. 8.
    Guyenet, P., Lefresne, P., Bossier, J., Beaujouan, J. C. and Glowinski, J. (1973): Molec. Pharmacol. 9:630–639.Google Scholar
  9. 9.
    Hamburger, V. and Hamilton, H.L. (1951): J. Morphol. 88:49–92.CrossRefGoogle Scholar
  10. 10.
    Haga, T. and Noda, H. (1973): Biochim. Biophys. Acta 291:564–575.Google Scholar
  11. 11.
    Katz, B. (1966): Nerve, Muscle and Synapses, McGraw-Hill, New York.Google Scholar
  12. 12.
    Macintosh, F. (1977): This volume.Google Scholar
  13. 13.
    Landmesser, L. and Pilar, G. (1974): J. Physiol. (Lond.) 241: 737–749.Google Scholar
  14. 14.
    Mürrin, L. C. and Kuhar, M.J. (1976): Molec. Pharmacol. 12:1082–1090.Google Scholar
  15. 15.
    Potter, L.T. (1970): J. Physiol. (Lond.) 206:145–166.Google Scholar
  16. 16.
    Potter, L.T. and Murphy, W. (1967): Biochem. Pharmacol. 16: 1386–1388.PubMedCrossRefGoogle Scholar
  17. 17.
    Schultz, S. G. and Curran, P. F. (1970): Physiol. Bev. 59:637–718.Google Scholar
  18. 18.
    Simon, J.B., Atweh, W. and Kuhar, M.J. (1976): J. Neurochem. 26:909–922.PubMedCrossRefGoogle Scholar
  19. 19.
    Simon, J.B. and Kuhar, M.J. (1976): J. Neurochem. 27:93–99.PubMedCrossRefGoogle Scholar
  20. 20.
    Suszkiw, J., Beach, B. and Pilar, G. (1976): J. Neurochem. 26: 1123–1131.PubMedCrossRefGoogle Scholar
  21. 21.
    Suszkiw, J. and Pilar, G. (1976): J. Neurochem. 26:1133–1138.PubMedCrossRefGoogle Scholar
  22. 22.
    Thomas, B.C. (1972): Physiol. Bev. 52:563–594.Google Scholar
  23. 23.
    Yamamura, H.I. and Snyder, S.H. (1973): J. Neurochem. 21:1355–1374.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • G. Pilar
    • 1
  • R. Beach
    • 1
  • K. Vaca
    • 1
  • J. Suszkiw
    • 1
  1. 1.Physiology Section, Biological Sciences GroupUniversity of ConnecticutStorrsUSA

Personalised recommendations