Nerve Terminal Sacs from Torpedo Electric Organ: A New Preparation for the Study of Presynaptic Cholinergic Mechanisms at the Molecular Level
Much of contemporary knowledge concerning the general mechanisms involved in chemically mediated synaptic transmission is based on cholinergic transmission at the vertebrate neuromuscular junction. During the past two and one-half decades this synapse has been the subject of extensive, detailed investigations using mainly physiological, morphological and pharmacological techniques (27). The simple arrangement of a single neuronal type innervating a single effector cell type allows for interpretation of experimental findings uncomplicated by the presence of other synaptic contacts. For biochemical studies, however, the neuromuscular synapse is, for technical reasons, difficult to work with. The relatively small proportion of nervous tissue in muscle and the problems encountered in attempts to concentrate it from the bulk of the surrounding tissue (32) provide technical barriers of sufficient strength to have deterred many potential biochemical studies with this synapse. Instead, most of the detailed biochemistry of cholinergic transmission has come from work with mammalian brain where techniques for isolating synaptosomes and synaptic vesicles (31) have been of central importance.
KeywordsSynaptic Vesicle Phospholipase Activity Presynaptic Membrane Choline Uptake Cholinergic Transmission
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