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Aging of Neurons in Culture

  • F. Howard Schneider
  • Suzanne G. Rehnberg
  • Mark P. Bear
Part of the Advances in Behavioral Biology book series (ABBI, volume 23)

Abstract

Valuable information about cellular and biochemical aspects of aging has been obtained from studies with human and non-human dividing diploid cells in culture (see reviews by Hay, 1970; Hayflick, 1974; Littlefield, 1976 and Orgel, 1973). However, less work has been done using in vitro models to study cellular mechanisms of aging in post-mitotic differentiated cells, such as muscle and nerve. Varon (1975) has recently reviewed the nerve tissue preparations availabe which could possibly be used to study neuronal aging, including organ cultures, nerve tissue explants and reaggregating and dispersed cell cultures. These preparations are especially useful for morphological and electrophysiological studies, but are not as satisfactory for biochemical studies since they consist of a complex mixture of cell types. Furthermore, preparations obtained from mature or very old animals are less likely to yield functionally intact preparations since with age there is an increase in the difficulty of dissociating neuronal tissue into single cells. In view of the difficulties in obtaining preparations of pure nerve cells from animals, we have considered the possibility of using continuous lines of neuroblastoma cells for studies on neuronal aging.

Keywords

Acid Phosphatase Neuroblastoma Cell Sodium Butyrate Acetylcholinesterase Activity Mouse Neuroblastoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • F. Howard Schneider
    • 1
  • Suzanne G. Rehnberg
    • 1
  • Mark P. Bear
    • 1
  1. 1.Geriatric Research, Educational and Clinical CenterVeterans Administration HospitalBedfordUSA

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