Aging of Neurons in Culture
Valuable information about cellular and biochemical aspects of aging has been obtained from studies with human and non-human dividing diploid cells in culture (see reviews by Hay, 1970; Hayflick, 1974; Littlefield, 1976 and Orgel, 1973). However, less work has been done using in vitro models to study cellular mechanisms of aging in post-mitotic differentiated cells, such as muscle and nerve. Varon (1975) has recently reviewed the nerve tissue preparations availabe which could possibly be used to study neuronal aging, including organ cultures, nerve tissue explants and reaggregating and dispersed cell cultures. These preparations are especially useful for morphological and electrophysiological studies, but are not as satisfactory for biochemical studies since they consist of a complex mixture of cell types. Furthermore, preparations obtained from mature or very old animals are less likely to yield functionally intact preparations since with age there is an increase in the difficulty of dissociating neuronal tissue into single cells. In view of the difficulties in obtaining preparations of pure nerve cells from animals, we have considered the possibility of using continuous lines of neuroblastoma cells for studies on neuronal aging.
KeywordsAcid Phosphatase Neuroblastoma Cell Sodium Butyrate Acetylcholinesterase Activity Mouse Neuroblastoma
Unable to display preview. Download preview PDF.
- Bear, M.P. and Schneider, F.H. The effect of media pH on the rate of growth, neurite formation and acetylcholinesterase activity in mouse neuroblastoma cells in culture. J. Cellular Physiol., In press, 1976.Google Scholar
- Daems, W.T., Wisse, E. and Brederoo, P. Electron microscopy of the vacuolar apparatus in lysosomes. In: Biology and Pathology, Vol. 1, (Eds. J.T.D. Ingle and H.B. Fell), Am. Elsevier Pub. Co., New York, pp. 97–103, 1969.Google Scholar
- Dunham, L.J. and Stewart, H.L. A survey of transplantable and transmissible tumors. J. of the Nat. Cancer Inst. 13:1299–1377, 1953.Google Scholar
- Hay, R.J. Cell strain senescence in vitro: Cell culture anomaly or an expression of a fundamental inability of normal cells to survive and proliferate. In: Aging in Cell and Tissue Culture, pp. 7–24, 1970.Google Scholar
- Hayflick, L. Cytogerontology. In: Theoretical Aspect of Aging, (Ed. M. Rockstein), Academic Press, New York, pp. 83–103, 1974.Google Scholar
- Littlefield, J.W. Variation, Senescence and Neoplasia. Harvard University Press, Cambridge, MA., 1976.Google Scholar
- Nandy, K. and Schneider, F.H. Lipofuscin pigment formation in neuro-blastoma cells in culture. In: Neurobiology of Aging, (Eds. R. D. Terry and S. Gershon), Raven Press, New York, pp. 245–264, 1976.Google Scholar
- Siakotos, A.N. and Armstrong, D. Age pigment, a biochemical indicator of intracellular aging. In: Neurobiology of Aging, (Eds. J. M. Ordy and K.R. Brizzee), Plenum Press, New York, pp. 369–399, 1975.Google Scholar
- Soukapova, M., Holeckova, E. and Hnevkovsky, P. Changes of the latent period of explanted tissues during ontogenesis of aging. In: Cell and Tissue Culture, (Eds. E. Holeckova and V. J. Cristofalo), Plenum Press, New York, pp. 41–56, 1970.Google Scholar
- Thuillier, G., Rumpf, P. and Thuillier, J. Preparation et. etude pharmacologique preliminaire des esters dimethylaminoethyliques de divers acides agissant comme regulateurs de croissance des vegetaux. Compt. Rend. Heb. Sean. L’Acad. Sci. Paris 249:2081–2083, 1959.Google Scholar
- Varon, S. In vitro approaches to the study of neural tissue agingo In: Survey Report on the Aging Nervous System, (Ed. G.J. Maletta), U.S. Government Printing Office, Washington, D.C., pp. 59–76, 1975.Google Scholar