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Neuroanatomical Techniques for Neurobehavioral Research

  • Gary Lynch
  • Herbert Killackey
Part of the Advances in Behavioral Biology book series (ABBI, volume 12)

Abstract

The coupling of modern neuroanatomical techniques with more behaviorally oriented approaches to problems of behavior and the brain has been extremely rare. This is unfortunate, in light of the arguments which have surrounded the interpretations of the anatomical substrate which underlies effects noted in various lesion and stimulation experiments (for example, the hypothalamus and regulation of feeding behavior). In this chapter we will discuss some of the newer anatomical techniques and attempt to demonstrate their relevance to neurobehavioral research.

Keywords

AChE Activity Ammonium Sulfide Terminal Degeneration Anatomical Technique Cupric Sulphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Benevento, L.A. and Ebner, F.F. The areas and layers of corticocortical terminations in the visual cortex of the Virginia opossum. J. Comp. Neurol., 1971, 141, 157–190.PubMedCrossRefGoogle Scholar
  2. Clarke, E. and O’Malley, CD. The Human Brain and Spinal Cord. Univ. of Calif, Press Berkeley, 1968.Google Scholar
  3. Cowan, W.M., Gottlieb, D.I., Hendrickson, A., Price, J.L. and Woolsey, T.A. The autoradiographic demonstration of axonal connections in the central nervous system. Brain Res., 1972, 37, 21–51.PubMedCrossRefGoogle Scholar
  4. Fink, R.P. and Heimer, L. Two methods for selective silver impregnation of degenerating axons and their synaptic ends in the central nervous system. Brain Res., 1967, 4, 369–374.PubMedCrossRefGoogle Scholar
  5. Fuxe, K. and Ungerstadt, U. Fluorescent microscopy in neuroanatomy. S. Ebbessen and W. Nauta, Eds.). Contemporary Research in Neuroanatomy, Springer, New York, 1970, pp. 275–314.CrossRefGoogle Scholar
  6. Guillery, R.W. Light- and electron-microscopical studies of normal and degenerating axons. S. Ebbessen and W. Nauta, (Eds.). Contemporary Research in Neuroanatomy. Springer, New York, 1970, pp. 77–105.CrossRefGoogle Scholar
  7. Hand, D.J. and Morrison, A.R. Thalmocortical relationships in the somatic sensory system as revealed by silver impregnation techniques. Brain. Behav. Evol., 1972 5, 273–302.PubMedCrossRefGoogle Scholar
  8. Heimer, L. Selective silver impregnation of degenerating axoplasm, S. Ebbessen and W. Nauta, (Eds.). Contemporary Research in Neuroanatomy. Springer, New York, 1970, pp. 106–131.CrossRefGoogle Scholar
  9. Heimer, L. Bridging the gap between light and electron microscopy in the experimental tracing of fiber connections. S. Ebbessen and W. Nauta, (Eds,). Contemporary ResearcH in Neuroanatomy, Springer, New York, 1970, pp. 162–172CrossRefGoogle Scholar
  10. Humanson, G.L. Animal tissue techniques. Freeman, San Francisco, 1967. pp. 130.Google Scholar
  11. Kalil, R.E. Formation of new retino-geniculate connections in kittens after removal of one eye. Anat. Rec., 1972, 172, 339–340.Google Scholar
  12. Killackey, H. Projections of the ventral nucleus to neocortex in the hedgehog. Anat. Rec., 1972, 172, 345.Google Scholar
  13. Killackey, H. and Ebner, F.F. Two different types of thalmocortical projections to a single cortical area in mammals. Brain. Behav. Evol., 1972, 16, 141–169.CrossRefGoogle Scholar
  14. Killackey, H. Anatomical evidence for cortical subdivisions based on vertically discrete thalamic projections from the ventral posterior nucleus to cortical barrels in the rat. Brain Res., 1973, 51, 326–331.PubMedCrossRefGoogle Scholar
  15. Killackey, H. and Ebner, F.F. Convergent projection of three separate thalamic nuclei on to a single cortical area. Science, 1973, 179, 283–285.PubMedCrossRefGoogle Scholar
  16. Killackey, H., Lynch, G. and Dunwiddie, T. Extracellular labelling of neurons in somatic sensory cortex with horseradish peroxidase, Anat. Rec., 1974, 178, 390–391.Google Scholar
  17. Kristensson, K. and Olsson, Y. Retrograde axonal transport of protein. Brain Res., 1971, 29, 363–365.PubMedCrossRefGoogle Scholar
  18. LaVail, J.H. and LaVail, M.M. Retrograde axonal transport in the central nervous system, Science, 1972, 176, 1416–1417.PubMedCrossRefGoogle Scholar
  19. Lasek, R., Joseph, B.S. and Whitlock, D.G. Evaluation of a radioautographlc neuroanatomical tracing method. Brain Res., 1968, 8, 319–336.PubMedCrossRefGoogle Scholar
  20. Lewis, P.R. and Shute, C.C.D. The cholinergic limbic system: Projection to hippocampal formation, medial cortex, nuclei of the ascending cholinergic reticular system and the subfornical organ and supra-optic crest. Brain, 1967, 90, 521–537.PubMedCrossRefGoogle Scholar
  21. Lund, R.D. and Lund, J.S. Reorganization of the retinotectal pathway in rats after neonatal retinal lesions. Exp. Neurol., 1973, 40, 337–390.Google Scholar
  22. Lynch, G., Lucas, P. and Deadwyler, S. The demonstration of acetylcholinesterase containing neurones within the caudate nucleus of the rat. Brain Res., 1972, 45, 617–621.PubMedCrossRefGoogle Scholar
  23. Lynch, G., Matthews, D., Mosko, S., Parks, T. and Cotman, C.W. Induced acetylcholinesterase-rich layer in rat dentate gyrus following entorhinal lesions. Brain Res., 1972, 42, 311–318.PubMedCrossRefGoogle Scholar
  24. Lynch, G., Smith, R.L., Mensah, P. and Cotman, C.W. Tracing the dentate gyrus mossy fiber system with horseradish peroxidase histochemistry. Exp. Neurol., 1973, 40, 516–524.PubMedCrossRefGoogle Scholar
  25. Lynch, G., Stanfield, B. and Cotman, C.W. Developmental differences in post-lesion axonal growth in the hippocampus. Brain Res., 1973, 59, 155–168.PubMedCrossRefGoogle Scholar
  26. Lynch, G., Mosko, S., Parks, T. and Cotman, C.W. Relocation and hyperdevelopment of the dentate gyrus commissural system after entorhinal lesions in immature rats. Brain Res., 1973, 50, 174–178.PubMedCrossRefGoogle Scholar
  27. Lynch, G., Gall, C., Mensah, P. and Cotman, C.W. Horseradish peroxidase histochemistry: A new method for tracing efferent projections in the central nervous system. Brain Res., 1974, 65, 373–380.PubMedCrossRefGoogle Scholar
  28. Lynch, G., Deadwyler, S.A. and Gall, C. Labeling of central nervous system neurones with extracellular recording microelectrodes. Brain Res., 1974, 66, 337–341.CrossRefGoogle Scholar
  29. Mosko, S., Lynch, G. and Cotman, C.W. Distribution of the septal projection to the hippocampal formation of the rat. J. Comp. Neurol., 1973, 152, 163–174.PubMedCrossRefGoogle Scholar
  30. Melgren, S.I. and Blackstad, T.W. Oxidative enzymes (tetrazoli-um reductases) in the hippocampal region of the rat, distribution and relation to architectonics. Z. Zellforsch., 1967, 78, 167–207.CrossRefGoogle Scholar
  31. Nauta, W.J.H. and Ryan, L.F. Selective silver impregnation of degenerating axons in the central nervous system. Stain Technol., 1952, 27, 175–179.PubMedGoogle Scholar
  32. Nauta, H.J.W. and Gygax, P.A. Silver impregnation of degenerating axons in the central nervous system: A modified technique. Stain Technol., 1954, 29, 91–93.PubMedGoogle Scholar
  33. Schneider, G.E. Mechanisms of functional recovery following lesions of visual cortex or superior colliculus in neonate and adult hamsters. Brain. Behav. Evol., 1970, 3, 295–323.PubMedCrossRefGoogle Scholar
  34. Shute, C.C.D. and Lewis, P.R. The use of Cholinesterase techniques combined with operative procedures to follow nervous pathways in the brain. In H.G. Schwarzacher (Ed.). Biblio, Anatomica, F.2 - Histochemsitry of Cholinesterase. Karger, New York, 1961, 34–39.Google Scholar
  35. Siegel, A., Bandler, R. and Flynn, J.P. Thalamic sites and pathways related to elicited attack. Brain. Behav. Evol., 1972, 6, 542–555.PubMedCrossRefGoogle Scholar
  36. Steward, O., Cotman, C.W. and Lynch, G. The nature of increased histochemical deposition of INT formazan in fields of degenerating synaptic terminals. Brain Res., 1973, 63, 183–193.PubMedCrossRefGoogle Scholar
  37. Steward, O., Lynch, G. and Cotjnan, C. Histochemical detection of orthograde degeneration in the central nervous system of the rat. Brain Res., 1973, 54, 65–73.PubMedCrossRefGoogle Scholar
  38. Steward, O., Cotman, C.W. and Lynch, G. Growth of a new fiber projection in the brain of adult rats: Re-innervation of the dentate gyrus by the contralateral entorhinal cortex following ipsllateral entorhina,l lesions, Exp. Brain Res,, In press.Google Scholar
  39. Strick, P.O. Cortical projections of the feline thalamic nucleus ventralis lateralus. Brain Res., 1970, 20, 130–134.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Gary Lynch
    • 1
  • Herbert Killackey
    • 1
  1. 1.University of CaliforniaIrvineUSA

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