Comparative Biochemical and Behavioral Studies of Chronic Schizophrenic Patients and Normals

  • H. E. Himwich
  • N. Narasimhachari
Part of the Advances in Behavioral Biology book series (ABBI, volume 10)


The pathfinding experiments of Kety and his co-workers (Pollin, Cardon and Kety, 1961) revealed that the administration of amino acids, including methionine or tryptophan, with a monoamine oxidase inhibitor, iproniazid (Marsilid), caused behavioral worsening, representing either a “biochemically-induced acute flare-up of a chronic schizophrenic process on the one hand, or a toxic delirium superimposed upon chronic schizophrenia on the other.” We used their method but gave the patients L-cysteine as the amino acid and tranylcypromine (Parnate) as the monoamine oxidase inhibitor. We added an additional facet by determining the changes in the urinary contents of these patients that took place simultaneously with the worsening of the symptoms. In all observations, psycho-pharmacological agents were replaced by similar looking placebos. All diagnoses were made according to the Diagnostic and Statistical Manual of Mental Disorders prepared by a committee of the American Psychiatric Association.


Urine Sample Schizophrenic Patient Tertiary Amine Monoamine Oxidase Inhibitor Chronic Schizophrenic Patient 
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  1. Axelrod, J., 1961, Enzymatic formation of psychotomimetic metabolites from normally occurring compounds, Science 134: 343.PubMedCrossRefGoogle Scholar
  2. Baumann, P., and Narasimhachari, N., 1973, Identification of N:N-dimethyltryptamine, 5-methoxy-N:N-dimethyltryptamine and bufotenin by cellulose TLC, J. Chromatog. 86: 269.CrossRefGoogle Scholar
  3. Baumann, P., Scherer, B., Kramer, W., and Matussek, N., 1971, Separation of indole derivatives and catecholamines by thin- layer chromatography, J. Chromatogr. 59: 463.PubMedCrossRefGoogle Scholar
  4. Capella, P., and Horning, E. C., 1966, Separation and identification of derivatives of biologic amines by gas-liquid chromatography, Anal. Chem. 38: 316.PubMedCrossRefGoogle Scholar
  5. “Diagnostic and Statistical Manual of Mental Disorders,” Second Edition, 1968, The Committee on Nomenclature and Statistics of the American Psychiatric Association, American Psychiatric Association, Washington, D. C.Google Scholar
  6. Gross, H., and Franzen, Fr., 1964, Zur Bestimmung körpereigener Amine in biologischen Substraten, Biochem. Z. 340: 403.PubMedGoogle Scholar
  7. Mandell, A. J., and Morgan, M., 1971, Indole(ethyl)amine N-methyl-transferase in human brain, Nature, (New Biology), 230: 85.Google Scholar
  8. Morgan, M., and Mandell, A. J., 1969, Indole(ethyl)amine N-methyl-transferase in the brain, Science 165: 492.PubMedCrossRefGoogle Scholar
  9. Narasimhachari, N., and Himwich, H. E., 1972, The determination of bufotenin in urine of schizophrenic patients and normal controls, J. Psychiat. Research 9: 113.CrossRefGoogle Scholar
  10. Narasimhachari, N., and Himwich, H. E., 1973, GC-MS identification of bufotenin in urine samples from patients with schizophrenia or infantile autism, Life Sei. 12 (Part II): 475.Google Scholar
  11. Narasimhachari, N., and Himwich, H. E., GC-MS identification of DMT in urine samples from drug-free chronic schizophrenic patients and its quantitation by the technique of single (selective) ion monitoring, Biochem. Biophys. Res. Commun, in press.Google Scholar
  12. Narasimhachari, N., Heller, B., Spaide, J., Haskovec, L., Fujimori, M., Tabushi, K., and Himwich, H. E., 1971a, Urinary studies of schizophrenics and controls, Biol. Psychiat. 3: 9.PubMedGoogle Scholar
  13. Narasimhachari, N., Plaut, J., and Leiner, K., 1971b, Thin-layer and gas-liquid chromatographic methods for the identification and estimation of indoleamines in urine samples, Biochem. Med. 5: 304.CrossRefGoogle Scholar
  14. Narasimhachari, N., Spaide, J., and Heller, B., 1971c, Gas liquid chromatographic and mass spectrometric studies on trimethylsilyl derivatives of N-methyl- and N,N-dimethyltryptamines, J. Chromatog. Sci. 9: 502.Google Scholar
  15. Narasimhachari, N. Avalos, J., Fujimori, M., and Himwich, H. E., 1972, Studies of drug-free schizophrenics and controls, Biol. Psychiat. 5: 311.Google Scholar
  16. Narasimhachari, N., Baumann, P., Pak, H. S., Carpenter, W. T., Zocchi, A. F., Hokanson, L., Fujimori, M., and Himwich, H. E., Gas chromatographic-mass spectrometric identification of urinary bufotenin and dimethyltryptamine in drug-free chronic schizophrenic patients, Biol. Psychiat. in press.Google Scholar
  17. Pollin, W., Cardon, P. V., Jr., and Kety, S. S., 1961, Effects of amino acid feedings in schizophrenic patients treated with iproniazid, Science 133: 104.PubMedCrossRefGoogle Scholar
  18. Rockland, L. H., and Pollin, W., 1965, Quantification of psychiatric mental status, Arch. Gen. Psychiat. 12: 23.PubMedCrossRefGoogle Scholar
  19. Saavedra, J. M., and Axelrod, J., 1972, Psychotomimetic N-methylated tryptamines: Formation in brain in vivo and in vitro, Science 175: 1365.PubMedCrossRefGoogle Scholar
  20. Saavedra, J. M., Coyle, J. T., and Axelrod, J., 1973, The distribution and properties of the nonspecific N-methyltransferase in brain, J. Neurochem. 20: 743.PubMedCrossRefGoogle Scholar
  21. Shulgin, A., 1970, Discussion, in “Psychotomimetic Drugs”, D. H. Efron ed., p. 119, Raven Press, New York.Google Scholar
  22. Snyder, S. H., and Richelson, E., 1968, Psychedelic drugs: Steric factors that predict psychotropic activity, Proc. Nat. Acad. Sci. U.S. 60: 206.CrossRefGoogle Scholar
  23. Szara, S., 1956, Dimethy1tryptamin: Its metabolism in man; the relation of its psychotic effect to the serotonin metabolism, Experientia 12: 441.PubMedCrossRefGoogle Scholar
  24. Szara, S., 1961, Hallucinogenic effects and metabolism of tryptamine derivatives in man, Fed. Proc. 20: 885.Google Scholar
  25. Szara, S., 1967, Hallucinogenic amines and schizophrenia (with a brief addendum on N-dimethyltryptamine), in “Amines and Schizophrenia,” H. E. Himwich, S. S. Kety, and J. R. Smythies eds., pp. 181–197, Pergamon Press, New York.Google Scholar
  26. Tanimukai, H., Ginther, R., Spaide, J., Bueno, J. R., and Himwich H. E., 1970, Detection of psychotomimetic N,N-dimethylated indoleamines in the urine of four schizophrenic patients, Brit. J. Psychiat. 117: 421.PubMedCrossRefGoogle Scholar
  27. Winer, B. J., 1962, Analysis of Variance, in “Statistical Principals in Experimental Design,” McGraw Hill, New York.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • H. E. Himwich
    • 1
  • N. Narasimhachari
    • 1
  1. 1.Thudichum Psychiatric Research LaboratoryGalesburg State Research HospitalGalesburgUSA

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