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Mammalian C-Type Oncornaviruses: Relationships between Viral Structural and Cell-Surface Antigens and Their Possible Significance in Immunological Defense Mechanisms

  • Werner Schäfer
  • Dani P. Bolognesi
Part of the Contemporary Topics in Immunobiology book series (CTI, volume 6)

Abstract

Over the past several years, there has been a coordinated effort between our research groups and those of P. Fischinger (Bethesda, Md.), F. de Noronha (Ithaca, N.Y.), and J. Ihle and M. Hanna, Jr. (Frederick Cancer Research Center) to study the various properties of mammalian C-type viruses. Our main intentions were: (1) To clarify the morphology of the virion; (2) to isolate its constituent proteins and glycoproteins; and (3) to characterize the structural components in biochemical, biological, and seroimmunological terms. In most of these studies, Friend leukemia virus (FLV) was employed as a model for murine viruses and the Rickard leukemia virus (FeLV) for feline agents. For both systems, suspension cultures were developed (Schäfer and Seifert, 1968; Seifert et al., 1975; Rickard et al., 1969) which produce the large quantities of virus necessary for this work. Other mammalian C-type viruses were also included in some of the studies dealing with viral structure and their serological relationship to the murine and feline viruses.

Keywords

Leukemia Virus Murine Leukemia Virus Endogenous Virus Major Glycoprotein Mouse Leukemia Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

FeLV

feline leukemia virus (Rickard)

FLV

Friend MuLV

GALV

gibbon ape lymphoma virus

GLV

Gross MuLV

gp45, gp 71, FLV

glycoproteins

HA

hemagglutination

IEM

immunoelectron microscopy

MuLV

murine leukemia virus

MuX

murine xenotropic C virus

PAS

periodic acid-Schiff reagents

p10–p31

FLV proteins (number indicates molecular weight X 103)

p.i.

post infection

RIA

radioimmunoassay

RLV

Rauscher MuLV

SDS PAGE

sodium dodecylsulfate—polyacrylamide gel electrophoresis

SSV-SSAV

simian sarcoma—simian sarcoma associated virus

UA

uranyl acetate staining

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Werner Schäfer
    • 1
  • Dani P. Bolognesi
    • 2
  1. 1.Max-Planck-Institut für VirusforschungTübingenGermany
  2. 2.Department of SurgeryDuke University Medical CenterDurhamUSA

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