Lysozymelike Activities in the Hemolymph of Crassostrea virginica

  • S. Y. Feng


In recent years the presence of lysozymelike activities in the hemolymph of invertebrates has been documented with increasing frequency. Mohrig and Messner (1968) report the occurrence of lysozyme at a level of 25–500 µg/ml in the hemolymph of normal Gallaria mellonella. This level can, however, be elevated nonspecifically up to a maximum of 9000 µg/ml of hemolymph within 24 hr by injections of sterile Ringer solution, India ink, and Gram-positive bacteria. Jolles and Zuili (1960) discovered a bacterial lytic factor in the marine polychaete worm, Nephthys hombergi, which Perin and Jolles (1972) characterize as lysozome. Schubert and Messner (1971) have also observed lysozymelike activities in nine species of marine, fresh-water, and terrestrial annelids: Arenicola marina, Nereis diversicolor, Tubifex sp., Enchytraeus sp., Allolobophora caligi- nosa, Hirudo medicinalis, Haemopis sanguisuga, Herpobdella octoculata, and Glossiphonia complanta. However, they do not observe heightened levels of lysozyme in these annelids after injection of various bacteria.


Basic Protein Crassostrea Virginica Papaya Latex Marine Science Institute Nereis Diversicolor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bonavida, B., Sapse, A. T., and Sercarz, E. E., 1967, Human tear lysozyme. I. Purification, physicochemical, and immunochemical characterization, J. Lab. Gin. Med. 70: 951–962.Google Scholar
  2. Feng, S. Y. and Canzonier, W. J., 1970, Humoral responses in the American oyster (Crassostrea virginica) infected with Bucephalus sp. and Minchinia nelsoni, in: A Symposium on Diseases of Fishes and Shellfishes, Special Pub. No. 5, pp. 497–510 (S. F. Snieszko, ed.), American Fisheries Society.Google Scholar
  3. Jolles, P., 1960, Lysozyme, in: The Enzymes, Vol. 4, 2nd ed., pp. 432–445 ( P. D. Boyer, H. Lardy, and K. Myrback, eds.), Academic Press, New York.Google Scholar
  4. Jolles, P. and Zuili, S., 1960, Purification et étude comparée de nouveaux lysozymes: extraits du poumon de poule et de Nephthys hombergi, Biochim. Biophys. Acta 39: 212–217.CrossRefGoogle Scholar
  5. McDade, J. E. and Tripp, M. R., 1967a, Lysozyme in the hemolymph of the oyster, Crassostrea virginica, J. Invertebr. Pathol. 9: 531–535.Google Scholar
  6. McDade, J. E. and Tripp, M. R., 1967b, Lysozyme in oyster mantle mucus, J. Invertebr. Pathol 9: 581–582.Google Scholar
  7. Messner, B. and Mohrig, W., 1969, Zum Lysozym-Vorkommen bei Muscheln, Anodonta anatina (L.), Zool. Jahrb. Physiol 74: 421–435.Google Scholar
  8. Meyer, K., Hohnel, E., and Steinberg, A., 1946, Lysozyme of plant origin, J. Biol Chem. 163: 733–740.PubMedGoogle Scholar
  9. Mohrig, W. and Messner, B., 1968, Immunreaktionen bei Insekten I. Lysozym als grundlegender antibakterieller Faktor im humoralen Abwehremechanismus der Insekten. Biol Zb., L’vivs’k. Derzh. Univ. 87: 439–470.Google Scholar
  10. Parry, Jr., R. M., Chandan, R. C., and Shahani, K. M., 1969, Isolation and characterization of human milk lysozyme, Arch. Biochem. Biophys. 103: 59–65.CrossRefGoogle Scholar
  11. Perin, J.-P. and Jolles, P., 1972, The lysozyme from Nephthys hombergi ( Annelid ), Biochim. Biophys. Acta 263. 683–689.Google Scholar
  12. Reisfeld, R. A., Lewis, U. J., and Williams, D. E., 1962, Disk electrophoresis of basic proteins and peptides on Polyacrylamide gels, Nature 195: (4838): 281–283.PubMedCrossRefGoogle Scholar
  13. Schubert, V. I. and Messner, B., 1971, Untersuchungen über das Vorkommen von Lysozym bei Anneliden, Zool. Jahrb. Physiol. 76: 36–50.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • S. Y. Feng
    • 1
    • 2
  1. 1.Marine Research LaboratoryUSA
  2. 2.Biological Sciences GroupUniversity of ConnecticutNoankUSA

Personalised recommendations