Antigen-Binding Receptors on Lymphocytes

  • Joseph M. Davie
  • William E. Paul


The concept that activation of immunocompetent cells by antigen depends upon the presence of specific antigen-binding receptors on the cell surface has been central to immunologic theory since it was originally proposed by Paul Ehrlich (1900). Direct evidence for the existence of such receptors has been amassed in recent years. In particular, it has been demonstrated that in nonimmunized animals, rare lymphocytes will bind a given substance to their surface and that the frequency of such cells increases upon immunization (Naor and Sulitzeanu, 1967; Byrt and Ada, 1969; Davie and Paul, 1971; Humphrey and Keller, 1970; Davie et al, 1971a).


Surface Immunoglobulin Mouse Spleen Cell Tolerant Mouse Clonal Selection Theory Nonimmune Animal 
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  1. Ada, G. L., Byrt, P., Mandel, T., and Warner, N., 1970. A specific reaction between antigen labelled with radioactive iodine and lymphocyte-like cells from normal, tolerant and immunized mice or rats. In J. Sterzl and I. Riha (eds.), Developmental Aspects of Antibody Formation and Structure, Vol. II, Academic Press, New York, p. 503.Google Scholar
  2. Ada, G. L., Nossal, G. J. V., and Pye, J., 1964. Antigens in immunity. III. Distribution of iodinated antigens following injection into rats via the hind footpads. Aust. J. Exp. Biol Med. Set 42: 295.CrossRefGoogle Scholar
  3. Bach, J. F., and Dardenne, M., 1972. Antigen recognition by T lymphocytes. I. Thymus and marrow dependence of spontaneous rosette-forming cells in the mouse. Cell. Immunol. 3: 1.PubMedCrossRefGoogle Scholar
  4. Breitner, J. C. S., 1973. Induction of hapten-specific tolerance in mouse B cells. Fed. Proc. 32: 1002 (abstr.).Google Scholar
  5. Burnet, F. M., 1959. The Clonal Selection Theory of Acquired Immunity. Cambridge University Press, Cambridge.Google Scholar
  6. Byrt, P., and Ada, G. L., 1969. An in vitro reaction between labelled flagellin or haemocyanin and lymphocyte-like cells from normal animals. Immunology 17: 503.PubMedGoogle Scholar
  7. Chiller, J. M., and Weigle, W. O., 1972. Cellular basis of immunological unresponsiveness. Contemp. Topics Immunobiol. 1: 119.Google Scholar
  8. Claflin, J. L., Lieberman, R., and Davie, J. M., 1974. Clonal nature of the immune response to phosphorylcholine. I. Specificity, class, and idiotype of phosphorylcholine binding receptors on lymphoid cells. J. Exp. Med. in press.Google Scholar
  9. Claflin, L., Merchant, B., and Inman, J., 1973. Antibody binding characteristics at the cellular level. I. Comparative maturation of hapten-specific IgM and IgG plaque- forming cell populations. J. Immunol. 110: 241.PubMedGoogle Scholar
  10. Cohen, B. E., Davie, J. M., and Paul, W. E., 1973. Hapten-specific tolerance: Depressed humoral and normal cellular immune responses. J. Immunol. 110: 213.PubMedGoogle Scholar
  11. Cooper, M. G., Ada, G. L., and Langman, R. E., 1972. The incidence of hemocyanin- binding cells in hemocyanin-tolerant rats. Cell Immunol 4: 289.PubMedCrossRefGoogle Scholar
  12. Cooper, M. D., Lawton, A. R., and Kincade, P., 1972. A developmental approach to the biological basis for antibody diversity. Contemp. Topics Immunobiol 1: 33.Google Scholar
  13. Crone, M., Koch, C., and Simonsen, M., 1972. The elusive T cell receptor. Transpl Rev. 10: 36.Google Scholar
  14. Davie, J. M., and Paul, W. E., 1973. Immunological maturation: Preferential proliferation of high affinity precursor cells. J. Exp. Med. 137: 201.PubMedCrossRefGoogle Scholar
  15. Davie, J. M., and Paul, W. E., 1972fl. Receptors on immunocompetent cells. V. Cellular correlates of the “maturation” of the immune response. J. Exp. Med. 135: 660.Google Scholar
  16. Davie, J. M., and Paul, W. E., 19726. Receptors on immunocompetent cells. IV. Direct measurement of avidity of cell receptors and cooperative binding of multivalent ligands. J. Exp. Med. 135: 643.Google Scholar
  17. Davie, J. M., and Paul, W. E., 1971. Receptors on immunocompetent cells. II. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 1251-binding lymphocytes of normal guinea pigs. J. Exp. Med. 134: 495.PubMedCrossRefGoogle Scholar
  18. Davie, J. M., Paul, W. E., and Asofsky, R., 1912a. Immunoglobulin class of receptors on DNP-specific antigen binding cells of guinea pigs. Fed. Proc. 31: 735 (abstr.).Google Scholar
  19. Davie, J. M., Paul, W. E., Katz, D. H., and Benacerraf, B., 1972b. Hapten-specific tolerance: Preferential depression of the high-affinity antibody response. J. Exp. Med. 136: 436.CrossRefGoogle Scholar
  20. Davie, J. M., Rosenthal, A. S., and Paul, W. E., 1971a. Receptors on immunocompetent cells. III. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 1251-binding lymphocytes of immunized guinea pigs. J. Exp. Med. 134: 517.CrossRefGoogle Scholar
  21. Davie, J. M., Paul, W. E., Mage, R., and Goldman, M. B., 1971b. Membrane-associated immunoglobulin of rabbit peripheral blood lymphocytes: Allelic exclusion at the b locus. Proc. Nat. Acad. Sci. 68: 430.Google Scholar
  22. Dwyer, J. M., Warner, N. L., and Mackay, I. R., 1972. Specificity and nature of the antigen-combining sites on fetal and mature thymus lymphocytes. J. Immunol 108: 1439.PubMedGoogle Scholar
  23. Ehrlich, P., 1900. On immunity with special reference to cell life. Proc. Roy. Soc. 66: 424.Google Scholar
  24. Eisen, H. N., and Siskind, G. W., 1964. Variations in affinities of antibodies during the immune response. Biochemistry 3: 996.PubMedCrossRefGoogle Scholar
  25. Herrod, H. G., and Warner, N. L., 1972. Inhibition by anti-/li chain sera of the cellular transfer of antibody and immunoglobulin synthesis in mice. J. Immunol 108: 1712.PubMedGoogle Scholar
  26. Hogg, N. M., and Greaves, M. F., 1972. Antigen-binding thymus-derived lymphocytes. II. Nature of the immunoglobulin determinants. Immunology 22: 967.PubMedGoogle Scholar
  27. Humphrey, J. H., and Keller, H. U., 1970. Some evidence for specific interaction between immunologically competent cells and antigens. In J. Sterzl, and I. Riha (eds.),Developmental Aspects of Antibody Formation and Structure, Vol. II, Academic Press, New York, p. 485.Google Scholar
  28. Julius, M. H., Masuda, T., and Herzenberg, L. A., 1972. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence- activated cell sorter. Proc. Nat. Acad. Sci. 69: 1934.PubMedCrossRefGoogle Scholar
  29. Katz, D. H., Davie, J. M., Paul, W. E., and Benacerraf, B., 1971. Carrier function in anti-hapten antibody responses. IV. Experimental conditions for the induction of hapten-specific tolerance or for the stimulation of anti-hapten anamnestic responses by “non-immunogenic” hapten-polypeptide conjugates. J. Exp. Med. 134: 201.PubMedCrossRefGoogle Scholar
  30. Kincade, P. W., and Cooper, M. D., 1971. Development and distribution of immunoglobulin- containing cells in the chicken. An immunofluorescent analysis using purified antibodies to µ, γ and light chains. J. Immunol. 106: 371.PubMedGoogle Scholar
  31. Kincade, P. W., Lawton, A. R., Bockman, D. E., and Cooper, M. D., 1970. Suppression of immunoglobulin G synthesis as a result of antibody-mediated suppression of immunoglobulin M synthesis in chickens. Proc. Nat. Acad. Sci. 67: 1918.PubMedCrossRefGoogle Scholar
  32. Klinman, N. R., 1971. Purification and analysis of “monofocal” antibody. J. Immunol. 106: 1345.PubMedGoogle Scholar
  33. Lamelin, J. P., Lisowska-Bernstein, B., Matter, A., Ryser, J. E., and Vasalli, P., 1972. Mouse thymus-independent and thymus-derived lymphoid cells. I. Immunofluorescent and functional studies. J. Exp. Med. 136: 984.PubMedCrossRefGoogle Scholar
  34. Lawton, A. R., Asofsky, R., Hylton, M. B., and Cooper, M. D., 1972. Suppression of immunoglobulin class synthesis in mice. I. Effects of treatment with antibody to M-chain. J. Exp. Med. 135: 277.PubMedCrossRefGoogle Scholar
  35. Makela, O., and Nossal, G. J. V., 1961. Bacterial adherence: A method for detecting antibody production by single cells. J. Immunol. 87: 447.PubMedGoogle Scholar
  36. Manning, D. D., and Jutilla, J. W., 1972. Immunosuppression of mice injected with heterologous anti-immunoglobulin heavy chain antisera. J. Exp. Med. 135: 1316.PubMedCrossRefGoogle Scholar
  37. Miller, A., DeLuca, D., Decker, J., Ezzell, R., and Sercarz, E. E., 1971. Specific binding of antigen to lymphocytes. Evidence for lack of unispecificity in antigen-binding cells. Am. J. Pathol. 65: 451.PubMedGoogle Scholar
  38. Moller, E., Sjoberg, O., and Makela, O., 1971. Immunological unresponsiveness against the 4-hydroxy-3,5-dinitro-phenacetyl (NNP) hapten in different lymphoid cell populations. Eur. J. Immunol. 1: 218.PubMedCrossRefGoogle Scholar
  39. Naor, D., and Sulitzeanu, D., 1969. Binding of 1251-BSA to lymphoid cells of tolerant mice. Intern. Arch. Allergy Appl. Immunol. 36: 112.CrossRefGoogle Scholar
  40. Naor, D., and Sulitzeanu, D., 1967. Binding of radioiodinated bovine serum albumin to mouse spleen cells. Nature 214: 687.PubMedCrossRefGoogle Scholar
  41. Nossal, G. J. V., Warner, N. L., Lewis, H., and Sprent, J., 1972. Quantitative features of a sandwich radioimmuno-labeling technique for lymphocyte surface receptors. J. Exp. Med. 135: 405.PubMedCrossRefGoogle Scholar
  42. Pasanen, V. J., and Makela, O., 1969. Effect of the number of haptens coupled to each erythrocyte on hemolytic plaque formation. Immunology 16: 399.PubMedGoogle Scholar
  43. Paul, W. E., 1970. Functional specificity of antigen-binding receptors of lymphocytes. Transpl. Rev. 5: 130.Google Scholar
  44. Pierce, C. W., Solliday, S. M., and Asofsky, R., 1912a. Immune responses in vitro. IV. Suppression of primary 7M, 7G, and 7A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to mouse immunoglobulin. J. Exp. Med. 135: 675.Google Scholar
  45. Pierce, C. W., Solliday, S. M., and Asofsky, R., 1912b. Immune responses in vitro. V. Suppression of 7M, 7G, and 7A plaque-forming cell responses in cultures of primed mouse spleen cells by class-specific antibody to mouse immunoglobulins. J. Exp. Med. 135: 698.Google Scholar
  46. Rabellino, E., Colon, S., Grey, H. M., and Unanue, E. R., 1971. Immunoglobulins on the surface of lymphocytes. I. Distribution and quantitation. J. Exp. Med. 133: 156.PubMedCrossRefGoogle Scholar
  47. Raff, M. C., 1970. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology 19: 637.PubMedGoogle Scholar
  48. Roelants, G. E., 1972. Quantitation of antigen specific T and B lymphocytes in mouse spleens. Nature New Biol. 236: 252.CrossRefGoogle Scholar
  49. Rosenthal, A. S., Davie, J. M., Rosenstreich, D. L., and Blake, J. T., 1972. Depletion of antibody-forming cells and their precursors from complex lymphoid populations. J. Immunol 108: 279.PubMedGoogle Scholar
  50. Schlesinger, M., 1970. Anti-0 antibodies for detecting thymus-dependent lymphocytes in the immune response of mice to SRBC. Nature 226: 1254.PubMedCrossRefGoogle Scholar
  51. Shevach, E., Green, I., Ellman, L., and Maillard, J., 1972. Heterologous antiserum to thymus-derived cells in the guinea pig. Nature New Biol 235: 19.PubMedGoogle Scholar
  52. Siskind, G. W., and Benacerraf, B., 1969. Cell selection by antigen in the immune response. Adv. Immunol 10: 1.PubMedCrossRefGoogle Scholar
  53. Sjoberg, O., and Moller, E., 1970. The presence of antigen-binding cells in tolerant animals. Nature 228: 780.PubMedCrossRefGoogle Scholar
  54. Takahashi, T., Old, L. J., Mclntire, K. R., and Boyse, E. A., 1971. Immunoglobulin and other surface antigens of cells of the immune system. J Exp. Med. 134: 815.PubMedCrossRefGoogle Scholar
  55. Warner, N. L., Byrt, P., and Ada, G. L., 1970. Blocking of the lymphocyte antigen receptor site with anti-immunoglobulin sera in vitro. Nature 226: 942.PubMedCrossRefGoogle Scholar
  56. Wilson, J. D., 1971. Immunoglobulin determinants on rosette-forming cells. Their changing nature during an immune response. Aust. J. Exp. Biol Med. Sci. 49: 415.PubMedCrossRefGoogle Scholar
  57. Zaalberg, O. B., van der Meul, V. A., and van Twisk, M. J., 1968. Antibody production by isolated spleen cells. A study of cluster and plaque techniques. J. Immunol 100: 451.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Joseph M. Davie
    • 1
    • 2
  • William E. Paul
    • 3
  1. 1.Department of PathologyWashington University School of MedicineSt. LouisUSA
  2. 2.Department of MicrobiologyWashington University School of MedicineSt. LouisUSA
  3. 3.Laboratory of Immunology National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA

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