Cholinergic Neurons: Septal-Hippocampal Relationships

  • Michael J. Kuhar


There is abundant evidence that acetylcholine functions as a central nervous system (CNS) neurotransmitter (Hebb, 1970). It is present throughout the CNS, although its concentration varies from region to region. There are reviews of the extensive biochemical studies of this compound (Hebb, 1972; Phillis, 1970a) and there have been numerous electrophysiological studies (Phillis, 1970b).


AChE Activity Cholinergic Neuron Choline Acetyltransferase Stratum Radiatum Stratum Pyramidale 
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  1. Amano, T., Richelson, E., And Nirenberg, M. Neurotransmitter Synthesis By Neuroblastoma Clones. Proceedings Of The National Academy Of Sciences, 1972, 69, 258–263.CrossRefGoogle Scholar
  2. Biscoe, T. J., And Straughan, D. W. Micro-Electrophoretic Studies Of Neurones In The Cat Hippocampus. Journal Of Physiology (London), 1966, 183, 341–359.Google Scholar
  3. Blackstadt, T. W., And Kjaerheim, A. Special Axo-Dendritic Synapses In The Hippocampal Cortex: Electron And Light Microscopic Studies On The Layer Of Mossy Fibers. Journal Of Comparative Neurology, 1961, 117, 133–146.CrossRefGoogle Scholar
  4. Browning, E. T. Fluorometric Assay For Choline And Acetylcholine. Analytical Biochemistry, 1972, 46, 624–638.PubMedCrossRefGoogle Scholar
  5. Burt, A. M. The Histochemical Demonstration Of Choline Acetyltransferase Activity In The Spinal Cord Of The Rat. Anatomical Record, 1969, 163, 162.Google Scholar
  6. Burt, A. M. A Histochemical Procedure For The Localization Of Choline Acetyltransferase Activity. Journal Of Histochemistry And Cytochemistry, 1970, 18, 408–415.PubMedCrossRefGoogle Scholar
  7. Chao, L. P., And Wolfgram, F. Purification And Some Properties Of Choline Acetyltransferase (E. C. 2. 3. 1. 6) from bovine brain. Journal of Neurochemistry, 1973, 20, 1075–1082.PubMedCrossRefGoogle Scholar
  8. Dahlstrom, A., And Fuxe, K. Evidence For The Existence Of Monoamine-Containing Neurons In The Central Nervous System. I. Demonstration Of Monoamines In The Cell Bodies Of Brain Stem Neurons. Acta Physiologica Scandinavica, 1962, Supplement 1965, 232, 1–55.Google Scholar
  9. Eng, L. F., Uyeda, C. T., Chao, L. P., And Wolfgram, Antibody To Bovine Choline Acetyltransferase And Immunofluorescent Localization Of The Enzyme In Neurons. Nature (London), 1974, 250, 243–245.CrossRefGoogle Scholar
  10. Falck, B., Hillarp, N., Thieme, G., And Torp, A. Fluorescence Of Catecholamines And Related Compounds Condensed With Formaldehyde. Journal Of Histochemistry And Cytochemistry, 1962, 10, 348–354.CrossRefGoogle Scholar
  11. Feigenson, M. E., And Saelens, J. K. An Enzyme Assay For Acetylcholine. Biochemical Pharmacology, 1969, 18, 1479–1486.PubMedCrossRefGoogle Scholar
  12. Fonnum, F. Topographical And Subcellular Localization Of Choline Acetyltransferase In The Rat Hippocampal Region. Journal Of Neurochemistry, 1970, 17, 1029–1037.PubMedCrossRefGoogle Scholar
  13. Goldberg, A. M., And Mccaman, R. E. A Quantitative Microchemical Study Of Choline Acetyltransferase And Acetylcholinesterase In The Cerebellum Of Several Species. Life Sciences, 1967, 6, 1493–1500.PubMedCrossRefGoogle Scholar
  14. Goldberg, A. M., And Mccaman, R. E. The Determination Of Picomole Amounts Of Acetylcholine In Mammalian Brain. Journal Of Neurochemistry, 1973, 20, 1–8.PubMedCrossRefGoogle Scholar
  15. Guerrero-Figueroa, R., Gonzalez, G., Barros, A., Guerrero-Figueroa, E., Debalbian Verster, F., And Heath, R. G. Cholinergic Synapes In The Hippocampus And Effects Of Topical Application Of Gaba Aminooxyacetic Acid And Acetylcholine On Hippocampal Epileptogenic Tissues Of The Cat. Acta Neurologica Latinoamericana, 1965, 11, 185–204.PubMedGoogle Scholar
  16. Hanin, I., And Jenden, D. Estimation Of Choline Esters In Brain By A New Gas Chromatographic Procedure. Biochemical Pharmacology, 1969, 18, 837–845.PubMedCrossRefGoogle Scholar
  17. Hebb, C. Cns At The Cellular Level: Identity Of Transmitter Agents. In V. E. Hall, A. C. Giese, And R. R. Sonnenschein ( Eds. ), Annual Review of Physiology, 1970, 32, 165–192.Google Scholar
  18. Hebb, C. Biosynthesis Of Acetylcholine In Nervous Tissue. Pharmacological Reviews, 1972, 52, 918–947.Google Scholar
  19. Jacobowitz, D., And Koelle, G. B. Histochemical Correlations Of Acetylcholinesterase And Catecholamines In Postganglionic Autonomic Nerves Of The Cat, Rabbit And Guinea-Pig. Journal Of Pharmacology And Experimental Therapeutics, 1965, 148, 225–237.PubMedGoogle Scholar
  20. Kasa, P., And Morris, D. Inhibition Of Choline Acetyltransferase And Its Histochemical Localization. Journal Of Neurochemistry, 1972, 19, 1299–1304.PubMedCrossRefGoogle Scholar
  21. Kasa, P., Mann, S. P., And Hebb, C. O. Localization Of Choline Acetyltransferase: Histochemistry At The Light Microscope Level. Nature (London), 1970a, 226, 812–814.CrossRefGoogle Scholar
  22. Kasa, P., Mann, S. P., And Hebb, C. O. Localization Of Choline Acetyltransferase: Ultrastructural Localization In Spinal Neurons. Nature (London), 19706, 226, 814–816.Google Scholar
  23. Koelle, G. G., And Friedenwald, J. S. A Histochemical Method For Localizing Cholinesterase Activity. Proceedings Of The Society For Experimental Biology And Medicine (New York), 1949, 70, 617–622.Google Scholar
  24. Kuhar, M. J., And Rommelspacher, H. Acetylcholinesterase-Staining Synaptosome From Rat Hippocampus: Relative Frequency And Tentative Estimation Of Internal Concentration Of Free Or “Labile Bound” Acetylcholine. Brain Research, 1974, 77, 85–96.PubMedCrossRefGoogle Scholar
  25. Kuhar, M. J., And Yamamura, H. Cholinergic Muscarinic Receptors In Rat Brain: Light Autoradiographic Localization By The Specific Binding Of A Potent Antagonist. Nature (London), 1975, 253, 560–561.CrossRefGoogle Scholar
  26. Kuhar, M. J., Sethy, V. H., Roth, R. H., And Aghajanian, G. K. Choline: Selective Accumulation By Central Cholinergic Neurons. Journal Of Neurochemistry, 1973, 20, 581–593.PubMedCrossRefGoogle Scholar
  27. Lewis, P. R., And Shute, C. C. D. The Cholinergic Limbic System: Brain, Journal Of Neurology, 1967, 90, 521–540.Google Scholar
  28. Lewis, P. R., Shute, C. C. D., And Silver, A. Confirmation From Choline Acetylase Of A Massive Cholinergic Innervation To The Rat Hippocampus. Journal Of Physiology (London), 1967, 191, 215–224.Google Scholar
  29. Lynch, G., Matthews, D. A., Masko, S., Parks, T., And Cotman, C. Induced Acetylcholinesterase-Rich Layer In Rat Dentate Gyrus Following Entorhinal Lesions. Brain Research, 1972, 42, 311–318.PubMedCrossRefGoogle Scholar
  30. Lynch, G. S., Masko, S., Parks, T., And Cotman, C. W. Relocation And Hyperdevelopment Of The Dentate Gyrus Commissural System After Entorhinal Lesions In Immature Rats. Brain Research, 1973, 50, 174–178.PubMedCrossRefGoogle Scholar
  31. Mcgeer, E. G., Wada, J. A., Terao, A., And Jung, E. Amine Synthesis In Various Brain Regions With Caudate Or Septal Lesions. Experimental Neurology, 1969, 24, 277–284.PubMedCrossRefGoogle Scholar
  32. Mcgeer, P. L., Mcgeer, E. G., Singh, V. K., And Chase, W. H. Choline Acetyltransferase Localization In The Central Nervous System By Immunohistochemistry. Brain Research, 1974, 81, 373–379.PubMedCrossRefGoogle Scholar
  33. Pepeu, G., Mulas, A., Ruffi, A., And Sotgiu, P. Brain Acetylcholine Levels In Rats With Septal Lesions. Life Sciences, 1971, 10, 181–184.CrossRefGoogle Scholar
  34. Phillis, J. W. The Metablolism Of Acetylcholine. In The Pharmacology Of Synapses. London: Pergamon Press, 1970A, Pp. 8–37.Google Scholar
  35. Phillis, J. W. Pharmacological Studies On Neurones In The Brain And Spinal Cord. Part I. Cholinergic Mechanisms. In The Pharmacology Of Synapses. London: Pergamon Press, 19706, Pp. 149–185.Google Scholar
  36. Reid, W. R., Haubrich, O. R., And Krishna, G. Enzymic Radioassay For Acetylcholine And Choline In Brain. Analytical Biochemistry, 1971, 42, 390–395.PubMedCrossRefGoogle Scholar
  37. Rommelspacher, H., And Kuhar, M. J. Effects Of Electrical Stimulation On Acetylcholine Levels In Central Cholinergic Nerve Terminals. Brain Research, 1974, 81, 243–251.PubMedCrossRefGoogle Scholar
  38. Rommelspacher, H., Goldberg, A. M., And Kuhar, M. J. Action Of Hemicholinium On Cholinergic Nerve Terminals After Alteration Of Neuronal Impulse Flow. Neuropharmacology, 1974, 13, 1015–1023.PubMedCrossRefGoogle Scholar
  39. Schmidt, D. E., Szilagyi, P. I. A., Alkon, D. L., And Green, J. P. A Method For Measuring Nanogram Quantities Of Acetylcholine By Pyrolysis-Gas Chromatography. Journal Of Pharmacology And Experimental Therapeutics, 1970, 174, 337–345.PubMedGoogle Scholar
  40. Sethy, V., Roth, R. H., Kuhar, M. J., And Van Woert, M. H. Choline And Acetylcholine: Regional Distribution And Effect Of Degeneration Of Cholinergic Nerve Terminals In The Rat Hippocampus. Neuropharmacology, 1973, 12, 819–824.PubMedCrossRefGoogle Scholar
  41. Shea, P. A., And Aprison, M. H. Levels Of Acetylcholine And Choline In Rat Brain As Determined By A Sensitive Two-Enzyme System. Federation Proceedings, 1973, 32, 430.Google Scholar
  42. Shute, C. C. D., And Lewis, P. R. Electron Microscopy Of Cholinergic Terminals And Acetylcholinesterase-Containing Neurons In Hippocampal Formation Of The Rat. Zeitschrift Fttr Zellforschung And Mikroskopische Anatomie, 1966, 69, 334–343.CrossRefGoogle Scholar
  43. Shute, C. C. D., And Lewis, P. R. The Ascending Cholinergic Reticular System. Brain; Journal Of Neurology, 1967, 90, 497–519.CrossRefGoogle Scholar
  44. Simon, J. R., Mittag, T. And Kuhar, M. J. The Inhibition Of Synaptosomal Uptake Of Choline By Various Choline Analogs. Biochemical Pharmacology, 1975, 24, 1139–1142.PubMedCrossRefGoogle Scholar
  45. Smith, C. M. The Release Of Acetylcholine From The Rabbit Hippocampus. British Journal Of Pharmacology, 1972, 45, 172.Google Scholar
  46. Srebro, B., Oderfeld-Nowak, B., Klodos, I., Dabrowska, J., And Narkiewicz, O. Changes In Acetylcholinesterase Activity In Hippocampus Produced By Septal Lesions In The Rat. Life Sciences, 1973, 12, 261–270.CrossRefGoogle Scholar
  47. Steiner, F. A. Influence Of Microelectrophoretically Applied Acetylcholine On Responsiveness Of Hip-Pocampal And Lateral Geniculate Neurons. Pfluegers Archiv; European Journal Of Physiology, 1968, 303, 173–180.CrossRefGoogle Scholar
  48. Storm-Mathisen, J. Quantitative Histochemistry Of Acetylcholinesterase In Rat Hippocampal Region Correlated To Histochemical Staining. Journal Of Neurochemistry, 1970, 17, 739–750.PubMedCrossRefGoogle Scholar
  49. Storm-Mathisen, J., And Fonnum, F. Neurotransmitter Synthesis In Excitory And Inhibitory Synapses Of Rat Hippocampus. In R. Paoletti, R. Fumagalli, And C. Galli (Eds.), Second International Meeting of the International Society for Neurochemistry. Milan: Tamburini, 1969, p. 382.Google Scholar
  50. Ungerstedt, U. Stereotoxic mapping of the monoamine pathways in the rat brain. Acta Physiologica Scandinavica Supplement, 1971, 267, 1–48.Google Scholar
  51. Yamamura, H., and Snyder, S. H. Choline: High-affinity uptake by rat brain synaptosomes. Science, 1972, 178, 626–628.Google Scholar
  52. Yamamura, H. I., Kuhar, M. J., and Snyder, S. H. In Vivo identification of muscarinic cholinergic receptor binding in rat brain. Brain Research, 1974, 80, 170–176.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Michael J. Kuhar
    • 1
  1. 1.Department of Pharmacology and Experimental Therapeutics and Psychiatry and the Behavioral SciencesJohns Hopkins University School of MedicineBaltimoreUSA

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