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Differential Scanning Calorimetry of Biological Membranes: Instrumentation

  • Peter J. Scheidler
  • Joseph M. Steim

Abstract

The ultimate goal of biological membrane studies is the elucidation of the structure and arrangement in situ of the various molecular species in the biological membrane as well as the correlation of this molecular organization with membrane function. This great enterprise is just aborning; only the most gross aspects of membrane architecture are understood today. There is general agreement, however, that one of the fundamental features of the membrane is an extensive lipid bilayer.

Keywords

Differential Scanning Calorimetry Differential Thermal Analysis Sample Holder Biological Membrane Sample Container 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ashe, G. B., and Steim, J. M., 1971, Membrane transitions in gram-positive bacteria, Biochem. Biophys. Acta 233:810.PubMedCrossRefGoogle Scholar
  2. Barrall, E. M., and Johnson, J. F., 1970, Differential scanning calorimetry theory and applications, in: Thermal Characterization Techniques (P. E. Slade and L. T. Jenkins, eds.), pp. 1–39, Dekker, New York.Google Scholar
  3. Blazyk, J. F., and Steim, J. M., 1972, Phàse transitions in mammalian membranes, Biochem. Biophys. Acta 266:737.PubMedCrossRefGoogle Scholar
  4. Delben, F., Crescenzi, V., and Quadrifoglio, F., 1969, A study of the thermal denaturation of ribonuclease by differential scanning calorimetry, Int. J. Protein Res. 1:145.PubMedCrossRefGoogle Scholar
  5. Freeberg, F. E., and Alleman, T. G., 1966, A sealed cell for use with a commercial scanning calorimeter, Anal. Chem. 38:1806.CrossRefGoogle Scholar
  6. Hoyer, H. W., and Barrett, E. J., 1966, Differential thermal analysis of mixtures of DNA and nucleosides, Anal. Biochem. 17:344.PubMedCrossRefGoogle Scholar
  7. Jackson, W. M., and Brandts, J. F., 1970, Thermodynamics of protein denaturation: A calorimetric study of the reversible denaturation of chymotrypsinogen and conclusions regarding the accuracy of the two-state approximation, Biochemistry 9:2294.PubMedCrossRefGoogle Scholar
  8. Karmas, E., and DiMarco, R. G., 1970, Water binding index of proteins as determined by differential microcalorimetry, in: Analytical Calorimetry (R. S. Porter and J. F. Johnson, eds.), pp. 135–145, Plenum Press, New York.Google Scholar
  9. Klump, H., Ackermann, T., and Neamann, E., 1969, Experimental thermodynamics of helix-random coil transition. II. Calorimetric measurement of transition enthalpies of poly A in acid aqueous solution. Biopolymers 7:423.CrossRefGoogle Scholar
  10. Mackenzie, R. C., 1970, Differential Thermal Analysis, Academic Press, New York.Google Scholar
  11. Murphy, C. B., 1974, Thermal analysis, Anal. Chem. 46:415R.CrossRefGoogle Scholar
  12. Olafsson, P. B., and Bryan, A. M., 1970, Evaluation of thermal decomposition temperatures of amino acids by differential ethalpic analysis, Mikrochim. Acta, p. 871.Google Scholar
  13. O’Neill, M. J., 1964, Analysis of a temperature controlled scanning calorimeter, Anal. Chem. 36:1238.CrossRefGoogle Scholar
  14. Perkin-Elmer Corporation, 1970, Bibliography of Differential Scanning Calorimetry, Norwak, Conn., May.Google Scholar
  15. Porter, R. S., and Johnson, J. F., eds., 1968, Analytical Calorimetry, Vol. 1, Plenum Press, New York.Google Scholar
  16. Porter, R. S., and Johnson, J. F., eds., 1970, Analytical Calorimetry, Vol. 2, Plenum Press, New York.Google Scholar
  17. Porter, R. S., Barrall, E. M., and Johnson, J. F., 1969, Thermodynamic order in mesophases, Accounts Chem. Res. 2:53.CrossRefGoogle Scholar
  18. Puett, D., 1967, DTA and heats of hydration of some polypeptides, Biopolymers 5:327.CrossRefGoogle Scholar
  19. Smothers, W. J., and Chiang, Y., 1966, Handbook of Differential Thermal Analysis, Chemical Publishing, New York.Google Scholar
  20. Steim, J. M., 1965, Differential thermal analysis of protein denaturation in solution, Arch. Biochem. Biophys. 112:599.PubMedCrossRefGoogle Scholar
  21. Steim, J. M., Tourtellotte, M. E., Reinert, J. C., McElhaney, R. N., and Rader, R. L., 1969, Calorimetric evidence for the liquid crystalline state of lipids in a biomembrane, Proc. Natl. Acad. Sci. 63:104.PubMedCrossRefGoogle Scholar
  22. Tsong, T. Y., Hearn, R. P., Wrathall, D. P., and Sturtevant, J. M., 1970, A calorimetric study of thermally induced conformational transitions in ribonuclease A and certain of its derivatives, Biochemistry 9:2666.PubMedCrossRefGoogle Scholar
  23. Watson, E. S., O’Neill, M. J., Justin, J., and Brenner, N., 1964, A differential scanning calorimeter for quantitative differential thermal analysis, Anal. Chem. 36:1238.CrossRefGoogle Scholar
  24. Weissberger, A., and Rossiter, B. W., 1971, Techniques of Chemistry, Vol. I, Part V, Wiley-Interscience, New York.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Peter J. Scheidler
    • 1
  • Joseph M. Steim
    • 1
  1. 1.Chemistry DepartmentBrown UniversityProvidenceUSA

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