Clonal Lines of Glial Cells

  • S. E. Pfeiffer
Part of the Current Topics in Neurobiology book series (CTNB)


Extrapolations from gross brain biochemical studies to nervous system function at the cellular level have been extremely difficult because of the complex interrelationships among a variety of neuronal and glial cell types. As a result, numerous attempts have been made to separate the cell types prior to biochemical analysis. For example, Lowry (1953), Hydén (1959), and Hamberger (1963) have hand-dissected glia from neurons in order to study their metabolic relationships, and Roots and Johnston (1965) have used a “sieving-fishing” method for their studies. However, although useful cell separations can be achieved this way, the yields are necessarily low. More recently, a variety of gradient centrifugation methods producing larger quantities of cells have been developed for preparing brain fractions enriched for neurons (Bocci, 1966; Satake and Abe, 1966; Satake et al., 1968; Flangas and Bowman, 1968; Sellinger et al., 1971), glia (Korey et al., 1958; Fewster et al., 1967), or both (Rose, 1967, 1969; Rose and Sinha, 1969; Freysz et al., 1968; Blomstrand and Hamberger, 1969; Norton and Poduslo, 1970). However, although these preparations have yielded valuable biochemical information, the enrichments are only partial and the cell viability is uncertain, thus precluding many important experiments.


Schwann Cell S100 Protein Myelin Basic Protein Experimental Allergic Encephalomyelitis Clonal Line 
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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • S. E. Pfeiffer
    • 1
  1. 1.Department of MicrobiologyUniversity of Connecticut Health CenterFarmingtonUSA

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