Differentiation of Aggregating Brain Cell Cultures

  • Nicholas W. Seeds
Part of the Current Topics in Neurobiology book series (CTNB)


Although nerve was the first tissue used for in vitro culture (Harrison, 1907), only recently have the techniques for the maintenance, growth, and development of nerve tissue in vitro advanced. Most studies of brain differentiation in vitro use small explants. These explants from various regions of fetal and newborn mouse brain can undergo structural and bioelectrical development during culture (Bornstein, 1964; Crain, 1966; Wolf, 1970). In addition, functional connections are formed between separated spinal cord, brain stem, and neocortex fragments (Crain et al., 1968). However, the absence of sensitive biochemical procedures for detecting picomoles of product formation, in addition to the small size of the explants, has primarily limited the study of biochemical differentiation in explants to histochemical observations (Hosli and Hosli, 1970).


Mouse Brain Fetal Brain Choline Acetyltransferase Glutamate Decarboxylase Adult Mouse Brain 


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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • Nicholas W. Seeds
    • 1
  1. 1.Department of Biophysics and Genetics and Department of PsychiatryUniversity of Colorado Medical CenterDenverUSA

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