The Role of DNA Polymerase I in Genetic Recombination and Viability of Escherichia coli

  • G. B. Smirnov
  • B. I. Sinzinis
  • A. S. Saenko
Part of the Basic Life Sciences book series

Abstract

The rate of formation of high-molecular-weight daughter DNA in the conditionally lethal double mutant polA12 uvrE502, incubated at nonpermissive temperature, was slower than that in the single polA12 mutant. There exist at least two pathways determining viability of Escherichia coli cells: one of them is dependent on polA + and recB + genes, while another is polA recB independent but requires the uvrE + gene and can be blocked by exonuclease I. The RecF but not the RecBC pathway of genetic recombination was found to be absolutely dependent on the polymerizing activity of DNA polymerase I. The involvement of DNA polymerase I in genetic recombination in the recB C sbsB strain and viability in the uvrE or recB strains suggest the existence of the common steps required for the accomplishing of the RecF pathway of recombination and for viability of E. coli.

Keywords

Sucrose Recombination Sedimentation Thymidine Dine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cooper, P. K. and Hanawalt, P. C. (1972). Proc. Nat. Acad. Sci. U.S.A. 65, 1156.CrossRefGoogle Scholar
  2. Gross, J. D. and Gross, M. (1969). Nature 224, 1166.PubMedCrossRefGoogle Scholar
  3. Gross, J. D., Grunstein, J. and Witkin, E. M. (1971). J. Mol. Biol. 58, 631.PubMedCrossRefGoogle Scholar
  4. Horii, Z.-I. and Clark, A. J. (1973). J. Mol. Biol. 80, 327.PubMedCrossRefGoogle Scholar
  5. Kanner, L. and Hanawalt, P. C. (1970). Biochem. Biophys. Res. Commun. 39, 149.PubMedCrossRefGoogle Scholar
  6. Kaplan, H. S. (1966). Proc. Nat. Acad. Sci. U.S.A. 55, 1442.CrossRefGoogle Scholar
  7. Mattern, I. E. (1971). In First European Biophysical Congress, Baden, Austria, p. 237.Google Scholar
  8. Monk, M. and Kinross, J. (1972). J. Bacteriol. 109, 971.PubMedGoogle Scholar
  9. Saenko, A. S., Sinzinis, B. I. and Smirnov, G. B. (1972). Mol. Biol. (USSR) 6, 655.Google Scholar
  10. Shizuya, H. and Dykhuizen, D. (1972). J. Bacteriol. 112, 676.PubMedGoogle Scholar
  11. Sinzinis, B. I., Smirnov, G. B. and Saenko, A. S. (1973). Biochem. Biophys. Res. Commun. 53, 309.PubMedCrossRefGoogle Scholar
  12. Smirnov, G. B. (1974). Genetika USSR 10, 102.Google Scholar
  13. Smirnov, G. B., Filkova, E. V. and Skavronskaya, A. G. (1973a) J. Gen. Microbiol. 76, 407.PubMedGoogle Scholar
  14. Smirnov, G. B., Filkova, E. V. and Skavronskaya, A. G. (1973b). Mol. Gen. Genet. 126, 225.CrossRefGoogle Scholar
  15. Smirnov, G. B., Filkova, E. V. and Skavronskaya, A. G., Saenko, A. S. and Sinzinis, B. I. (1973c). Mol. Gen. Genet. 121, 139.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • G. B. Smirnov
    • 1
  • B. I. Sinzinis
    • 2
  • A. S. Saenko
    • 2
  1. 1.The Gamaleya Institute for Epidemiology and MicrobiologyAMS USSRMoscowUSSR
  2. 2.Research Institute of Medical RadiologyAMS USSRObninsk, Kaluga RegionUSSR

Personalised recommendations