The Two-Lesion Hypothesis for UV-Induced Mutation in Relation to Recovery of Capacity for DNA Replication

  • Charles O. Doudney
Part of the Basic Life Sciences book series


Evidence has accumulated (Doudney, 1961, 1963, 1965, 1966, 1968, 1969; Doudney and Young, 1962) that supports the hypothesis that in Escherichia coli two different UV-induced lesions are necessary for induction of mutations. This hypothesis was prompted by the dose-squared response to UV seen with mutation in bacteria. The evidence suggested that the two different lesions responsible for mutation are a pyrimidine dimer within the potentially mutated gene length together with a “DNA-synthesis blocking lesion” bringing a requirement for RNA and protein synthesis if recovery of DNA replication (and mutation) is to occur.


Pyrimidine Dimer Prophage Induction Albert Einstein Medical Initiation Macro Postirradiation Incubation 


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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Charles O. Doudney
    • 1
  1. 1.Department of GeneticsAlbert Einstein Medical CenterPhiladelphiaUSA

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