The Two-Lesion Hypothesis for UV-Induced Mutation in Relation to Recovery of Capacity for DNA Replication
Evidence has accumulated (Doudney, 1961, 1963, 1965, 1966, 1968, 1969; Doudney and Young, 1962) that supports the hypothesis that in Escherichia coli two different UV-induced lesions are necessary for induction of mutations. This hypothesis was prompted by the dose-squared response to UV seen with mutation in bacteria. The evidence suggested that the two different lesions responsible for mutation are a pyrimidine dimer within the potentially mutated gene length together with a “DNA-synthesis blocking lesion” bringing a requirement for RNA and protein synthesis if recovery of DNA replication (and mutation) is to occur.
KeywordsPyrimidine Dimer Prophage Induction Albert Einstein Medical Initiation Macro Postirradiation Incubation
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