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Replication and Expression of Constructed Plasmid Chimeras in Transformed Escherichia coli—A Review

  • Stanley N. Cohen
  • Annie C. Y. Chang
Part of the Basic Life Sciences book series

Abstract

EcoRI restriction-endonuclease-generated fragments of bacterial plasmids isolated from Staphylococcus aureus or Escherichia coli, or of amplified DNA coding for the 18S and 28S ribosomal RNA of Xenopus laevis, have been linked to the pSC 101 plasmid replicon and introduced into E. coli by transformation. The constructed plasmid chimeras can be cloned as stable replicons in E. coli, where they synthesize RNA and/or protein products specified by their component genes.

Keywords

Buoyant Density Bacterial Plasmid Cesium Chloride Gradient Plasmid Replicon Intact Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Stanley N. Cohen
    • 1
  • Annie C. Y. Chang
    • 1
  1. 1.Department of MedicineStanford University School of MedicineStanfordUSA

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