Analysis of Temperature-Sensitive recB and recC Mutations
The in vivo pleiotropic effects associated with the temperature-sensitive recB270 and recC271 mutations have been correlated with the in vitro behavior of the recBC nucleases coded for by these alleles. The ATP-dependent breakdown of double-stranded DNA is essential for cell viability, radiation repair, and genetic recombination. Temperature sensitivity can be suppressed in vitro and in vivo.
KeywordsTemperature Sensitivity Mutant Enzyme Genetic Recombination United States Public Health polA Mutation
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