Exonuclease VII of Escherichia coli

  • John W. Chase
  • Charles C. Richardson
Part of the Basic Life Sciences book series


A new exonuclease of Escherichia coli K12, exonuclease VII, has been purified 1700-fold and characterized. The enzyme is specific for single-stranded DNA and can initiate hydrolysis at both 5′ and 3′ termini. It is also capable of thyminedimer excision in vitro. The limit products of the reaction are oligonucleotides, predominantly in the range of tetramers to dodecamers. DNA is hydrolyzed by the enzyme in a processive fashion. Mutants of E. coli have been isolated having reduced levels of exonuclease VII activity in crude extracts. Mapping studies place the exonuclease VII locus between 45 and 56 minutes on the E. coli K12 linkage map.


Exonuclease Activity Endonuclease Activity Standard Reaction Mixture Thymine Dimer Alkaline Sucrose Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chase, J. W. and Richardson, C. C. (1974a). J. Biol. Chem. 249, 4545.PubMedGoogle Scholar
  2. Chase, J. W. and Richardson, C. C. (1974b). J. Biol. Chem. 249, 4553.PubMedGoogle Scholar
  3. Cooper, P. K. and Hanawalt, P. C. (1972). J. Mol. Biol. 67, 1.PubMedCrossRefGoogle Scholar
  4. Frenkel, G. D. and Richardson, C. C. (1971). J. Biol. Chem. 246, 4839.PubMedGoogle Scholar
  5. Kaplan, J. C., Kushner, S. R. and Grossman, L. (1971). Biochemistry 10, 3315.PubMedCrossRefGoogle Scholar
  6. Kushner, S. R., Kaplan, J. C., Ono, H. and Grossman, L. (1971). Biochemistry 10, 3325.PubMedCrossRefGoogle Scholar
  7. Milcarek, C. and Weiss, B. (1972). J. Mol. BioL. 68, 303.PubMedCrossRefGoogle Scholar
  8. Rupp, W. D. and Howard-Flanders, P. (1968). J. Mol. BioL. 31, 291.PubMedCrossRefGoogle Scholar
  9. Signer, E. (1971). In The Bacteriophage Lambda (Hershey, A. D., ed.), p. 139. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  10. Taylor, A. L. and Trotter, C. D. (1972). Bacteriol. Rev. 36, 504.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • John W. Chase
    • 1
  • Charles C. Richardson
    • 1
  1. 1.Department of Biological ChemistryHarvard Medical SchoolBostonUSA

Personalised recommendations