Involvement of Escherichia coli DNA Polymerase-I-Associated 5′→3′ Exonuclease in Excision-Repair of UV-Damaged DNA
From comparative studies between Escherichia coli Po1A107 cells (lacking 5′→3′ exonucleolytic activity associated with DNA polymerase I) and the isogenic wild-type strain, and between the purified DNA polymerise I preparations isolated from these strains, it can be concluded that the 5’→3’ exonuclease is involved in excision of pyrimidine dimers in E. coli. Evidence is presented that the polA107 mutation is located on that part of the DNA polymerase I gene coding for the small fragment on which 5′→3′ exonucleolytic activity is found.
KeywordsPhenyl Methane Sulfonylfluoride Pyrimidine Dimer polAl07 Mutation Methane Sulfonylfluoride Displace Strand
Unable to display preview. Download preview PDF.
- Boyle, J. M., Paterson, M. C. and Setlow, R. B. (1970). Nature 226, 708–710. Brutlag, D. L. and Kornberg, A. (1972). J. Biol. Chem. 247, 241–248.Google Scholar
- Cooper, P. K. and Hanawalt, P. C. (1972). Proc. Nat. Acad. Sci. U.S.A. 68, 2954–2957.Google Scholar
- Glickman, B. W. (1974). Biochim. Biophys. Acta 335, 115–122.Google Scholar
- Jovin, T. M., Englund, P. T. and Bertsch, L. L. (1969). J. Biol. Chem. 244, 2996–3008. Kato, T. and Kondo, S. (1970). J. Bacteriol. 104, 871–881.Google Scholar