Involvement of Escherichia coli DNA Polymerase-I-Associated 5′→3′ Exonuclease in Excision-Repair of UV-Damaged DNA

  • Herbert L. Heyneker
  • Hans Klenow
Part of the Basic Life Sciences book series


From comparative studies between Escherichia coli Po1A107 cells (lacking 5′→3′ exonucleolytic activity associated with DNA polymerase I) and the isogenic wild-type strain, and between the purified DNA polymerise I preparations isolated from these strains, it can be concluded that the 5’→3’ exonuclease is involved in excision of pyrimidine dimers in E. coli. Evidence is presented that the polA107 mutation is located on that part of the DNA polymerase I gene coding for the small fragment on which 5′→3′ exonucleolytic activity is found.


Phenyl Methane Sulfonylfluoride Pyrimidine Dimer polAl07 Mutation Methane Sulfonylfluoride Displace Strand 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Herbert L. Heyneker
    • 1
  • Hans Klenow
    • 2
  1. 1.Laboratory of Molecular GeneticsLeiden State UniversityLeidenThe Netherlands
  2. 2.Biokemiske Institut BUniversity of CopenhagenCopenhagen ØDenmark

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