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Two Temperature-Sensitive polA Mutants: An Approach to the Role in Vivo of DNA Polymerase I

  • Dennis G. Uyemura
  • E. B. Konrad
  • I. R. Lehman
Part of the Basic Life Sciences book series

Abstract

DNA polymerase I (Pol I) purified from a strain carrying polA12 demonstrates a defect primarily in the polymerase portion of the molecule. The mutant enzyme is altered in its structure and template specificity. Pol I isolated from strains carrying polAex1 are primarily defective in 5′→3′ exonuclease. The polAex1 lesion renders strains conditionally lethal.

Keywords

Polymerase Activity Mutant Enzyme Sucrose Density Gradient Exonuclease Activity Structural Perturbation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Glickman, B. W., van Sluis, C. A., Heijneker, H. L. and Rörsch, A. (1973). Mol. Gen. Genet. 124, 69–82.PubMedCrossRefGoogle Scholar
  2. Konrad, E. B. and Lehman, I. R. (1974). Proc. Nat. Acad. Sci. U.S.A. 71, 2048–2051.CrossRefGoogle Scholar
  3. Lehman, I. R. and Chien, J. R. (1973). J. Biol. Chem. 248, 7717–7723.Google Scholar
  4. Monk, M. and Kinross, J. (1972). J. Bacteriol. 109, 971–978.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Dennis G. Uyemura
    • 1
  • E. B. Konrad
    • 1
  • I. R. Lehman
    • 1
  1. 1.Department of BiochemistryStanford University School of MedicineStanfordUSA

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