Two Temperature-Sensitive polA Mutants: An Approach to the Role in Vivo of DNA Polymerase I
DNA polymerase I (Pol I) purified from a strain carrying polA12 demonstrates a defect primarily in the polymerase portion of the molecule. The mutant enzyme is altered in its structure and template specificity. Pol I isolated from strains carrying polAex1 are primarily defective in 5′→3′ exonuclease. The polAex1 lesion renders strains conditionally lethal.
KeywordsPolymerase Activity Mutant Enzyme Sucrose Density Gradient Exonuclease Activity Structural Perturbation
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- Lehman, I. R. and Chien, J. R. (1973). J. Biol. Chem. 248, 7717–7723.Google Scholar