Substrate Specificity of Micrococcus luteus UV Endonuclease and Its Overlap with DNA Photolyase Activity
The action of an endonuclease from Micrococcus luteus that operates on UV damage in DNA overlaps with that of DNA photolyase from yeast: homo- and heterocyclobutane dipyrimidines in DNA are substrates for both enzymes, but pyrimidine adducts or the “spore photoproduct” in DNA are not. As expected from this overlap, the action of the two enzymes is mutually interfering: single-strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photolyase-cyclobutane dipyrimidine complex can prevent nicking by the endonuclease.
KeywordsMicrococcus Luteus Spore Photoproduct Alkaline Sucrose Gradient Incision Step Sucrose Gradient Sedimentation
Unable to display preview. Download preview PDF.
- Patrick, M. H. and Harm, W. (1971). Biophys. Soc. Abstr. 11, 194.Google Scholar