Substrate Specificity of Micrococcus luteus UV Endonuclease and Its Overlap with DNA Photolyase Activity

  • M. H. Patrick
Part of the Basic Life Sciences book series


The action of an endonuclease from Micrococcus luteus that operates on UV damage in DNA overlaps with that of DNA photolyase from yeast: homo- and heterocyclobutane dipyrimidines in DNA are substrates for both enzymes, but pyrimidine adducts or the “spore photoproduct” in DNA are not. As expected from this overlap, the action of the two enzymes is mutually interfering: single-strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photolyase-cyclobutane dipyrimidine complex can prevent nicking by the endonuclease.


Micrococcus Luteus Spore Photoproduct Alkaline Sucrose Gradient Incision Step Sucrose Gradient Sedimentation 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • M. H. Patrick
    • 1
  1. 1.Institute for Molecular BiologyThe University of Texas at DallasDallasUSA

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