Purifying the Escherichia coli Photoreactivating Enzyme

  • Betsy M. Sutherland
Part of the Basic Life Sciences book series


Purification of the photoreactivating enzyme from Escherichia coli depends on three major factors: (1) development of a suitable assay, (2) selection of a source with high enzyme levels, and (3) development of a gentle, specific purification procedure. The preparation of purified enzyme is useful both in study of DNA repair enzymes and in the analysis of the role of cyclobutyl pyrimidine dimers in the induction of biological damage by ultraviolet light.


Biological Damage Suitable Assay High Enzyme Level Nuclease Sensitivity Impure Preparation 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Betsy M. Sutherland
    • 1
  1. 1.Department of Molecular Biology and BiochemistryUniversity of CaliforniaIrvineUSA

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