An Enzymatic Assay for Pyrimidine Dimers in DNA

  • Ann Ganesan
Part of the Basic Life Sciences book series


An assay for pyrimidine dimers in DNA employing the T4 endonuclease V is described. The assay can detect approximately 50 dimers per Escherichia coli genome, or the effect of 10 ergs/mm2 ultraviolet irradiation.


Enzymatic Assay Ultraviolet Irradiation Fraction Number Irradiate Cell Mechanical Shear 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Friedberg, E. C. (1972). Mutat. Res. 15, 113–123.PubMedCrossRefGoogle Scholar
  2. Friedberg, E. C. and Clayton, D. A. (1972). Nature, 237, 99–100.PubMedCrossRefGoogle Scholar
  3. Friedberg, E. C., Minton, K., Pawl, G. and Verzola, P. (1974). J. Virol. 13, 953–959.PubMedGoogle Scholar
  4. Ganesan, A. K. (1973). Proc. Nat. Acad. Sci. U.S.A. 70, 2753–2756.CrossRefGoogle Scholar
  5. Ganesan, A. K. (1974). J. Mol. Biol. 87, 103–119.PubMedCrossRefGoogle Scholar
  6. Paterson, M. C., Lohman, P. H. M. and Sluyter, M. L. (1973). Mutat. Res. 19, 245–256.PubMedCrossRefGoogle Scholar
  7. Wilkins, R. J. (1973a). Biochim. Biophys. Acta, 372, 33–37.Google Scholar
  8. Wilkins, R. J. (1973b). Int. J. Radiat. Biol. 24, 609–613.CrossRefGoogle Scholar
  9. Yasuda, S. and Sekiguchi, M. (1970). Proc. Nat. Acad. Sci. U.S.A. 67, 1839–1845.CrossRefGoogle Scholar
  10. Yasuda, S. and Sekiguchi, M. (1970). Proc. Nat. Acad. Set U.S.A. 67, 1839–1845.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Ann Ganesan
    • 1
  1. 1.Department of Biological SciencesStanford UniversityStandfordUSA

Personalised recommendations