Comparative Aspects of the Biochemistry of Fertilization: Regulatory Mechanisms of DNA Synthesis

  • B. De Petrocellis
  • P. Grippo
  • A. Monroy
  • E. Parisi
  • M. Rossi
Part of the Basic Life Sciences book series (BLSC, volume 4)


The onset of the terminal phase of differentiation of the female germinal cell, the transition from oogonium to oocyte, is marked by a suppression of the ability of the cell to replicate its nuclear DNA and divide. After the developing cell undergoes the last oogonial mitotic division, the chromosomes enter the prophase of the first meiotic division, replicate their DNA, and attain the 4C condition. No further overall replication of the DNA takes place throughout the entire period of oocyte growth and until the egg is fertilized. During this long period of oogenesis, however, the oocyte genome is transcriptionally very active: messenger RNAs are synthesized in large amounts. Nevertheless, most of these direct gene products are not immediately translated but are stored in the cytoplasm of the oocyte in order to be available for protein synthesis during the early postfertilization stages of development, stages during which the very high replicative activity of the DNA virtually precludes transcriptional activity.


Thymidine Kinase Ribonucleotide Reductase DNase Activity Blastula Stage Thymidylate Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

















copolymer of deoxyadenosine monophosphate and deoxy thymidine monophosphate;


double-stranded homopolymers of adenosine monophosphate and deoxy thymidine monophosphate;


hybrid of the homopolymer polyrA and oligodeoxythymidine monophos­phate;


double-stranded homopolymers of deoxyguanosine mono­phosphate and deoxycytidine monophosphate;




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  1. Brachet, J. (1968), Some effects of deoxyribonucleosides on sea urchin egg development, CurnMod. Biol 1:314Google Scholar
  2. Burger, M. M. (1971), Cell Surfaces in Neoplastic Transformation Current Topics in CellularRegulation, Vol. 3, p. 135.Google Scholar
  3. Burger, M. M. (1973), Surface changes in transformed cells detected by lectins, Fed. Proc.32:91PubMedGoogle Scholar
  4. De Petrocellis, B., Grant, P., and Scarano, E. (1965), Deoxycytidylate aminohydrolase during embryonic development of Rana Esculenta, Bioch. Biophys. Acta 87:209Google Scholar
  5. De Petrocellis, B., and Parisi, E. (1972), Changes in alkaline deoxyribonuclease activity in sea urchin during embryonic development, Exp. Cell Res. 73:496PubMedCrossRefGoogle Scholar
  6. De Petrocellis, B., and Parisi, E. (1973a), Deoxyribonuclease in sea urchin embryos. Comparison of the Activity present in developing embryos, in Nuclei, and in Mitochon­dria,Exp. Cell Res. 79:53PubMedCrossRefGoogle Scholar
  7. De Petrocellis, B., and Parisi, E. (1973b) Effect of Actinomycin and Puromycin on the DNAse activity in sea urchin embryos, Abstract of 9th International Congress ofBiochemistry, Stockholm, 1–7 July, 3 m 35, pg. 185.Google Scholar
  8. De Petrocellis, B., and Parisi, E. (1973), Effect of Actinomycin and Puromycin on the deoxyribonuclease activity in P.lividus embryos at various stages of development, Exp.Cell Res.52:351.CrossRefGoogle Scholar
  9. De Petrocellis, B., and Rossi, M. (1974) (manuscript in preparation)Google Scholar
  10. De Vincentis, E., De Petrocellis, B., and Scarano, E. (1959), La deaminazione enzimatica delTacido 5′-deossicitidilico e dell’acido 5-metil-5′-deossicitidilico nella rigenerazione epatica, Boll Soc. Ital. Biol. Sper. 35:786Google Scholar
  11. Epel, D., Weaver, A. M., Muchmore, A. V., and Schimke, R. T. (1969), ß3-l–3-glucanase of sea urchin eggs: release from particles at fertilization, Science 163:294PubMedCrossRefGoogle Scholar
  12. Fansler, B., and Loeb, L. A. (1969), Sea urchin nuclear DNA polymerase. II.Changing localization during early development, Exp. Cell Res. 57:305PubMedCrossRefGoogle Scholar
  13. Fansler, B., and Loeb, L. A. (1972), Sea urchin nuclear DNA polymerase. IV. Reversible association of DNA polymerase with nuclei during the cell cycle, Exp. Cell Res. 75:433.PubMedCrossRefGoogle Scholar
  14. Fox, T. O., Sheppard, J. B., and Burger, M. M. (1971), Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis, Proc. Natl. Acad. Sei. USA 68:244CrossRefGoogle Scholar
  15. Giusti, G., Mangoni, C, De Petrocellis, B. and Scarano, E. (1970), Deoxycytidylate de­aminase and deoxycytidine deaminase in normal and neoplastic human tissues, E.Enzyme. Biol. Clin. 11:315Google Scholar
  16. Grippo, P., and Lo Scavo, A. (1972), DNA polymerase activity during maturation of Xenopus laevis oocytes, Bioch. Biophys. Res. Commun.45:280.CrossRefGoogle Scholar
  17. Grippo, P., Locorotondo, G., and Caruso, A. (1974), DNA polymerases from oocytes and eggs of Xenopus laevis: partial purification and characterization, in press.Google Scholar
  18. Grippo, P. (1973), Partial purification and properties of two DNA polymerase activities in eggs and oocytes, 9th International Congress of Biochemistry, Stockholm, p. 185.Google Scholar
  19. Gurdon, J. B. (1967), On the origin and persistence of a cytoplasmic state inducing nuclear DNA synthesis in frogs’ eggs,Prac. Natl. Acad. Sei. USA 58:545CrossRefGoogle Scholar
  20. Gurdon, J. B., Birnsteil, M. L., and Speight, V. A. (1969), The replication of purified DNA introduced into living egg cytoplasm, Biochem. Biophys. Acta 174:614.PubMedGoogle Scholar
  21. Hamburger, V. (1960), A Manual of Experimental Exmbryology, University Chicago Press, Chicago.Google Scholar
  22. Infante, A. A., Nauta, R., Gilbert, S., Hobart, P., and Firshein, W. (1973), DNA synthesis in developing sea urchins: role of a DNA nuclear membrane complex, Nature New Biol.242:5PubMedCrossRefGoogle Scholar
  23. Isono, N. (1963), Intracellular localization of enzymes of pentose phosphate cycle in unfertilized and fertilized eggs, J. Fac. Sei. Univ. Tokyo, Sect. 4, 10:37.Google Scholar
  24. Kleinschuster, S. J., and Moscona, A. A. (1972), Interactions of embryonic and fetal neural retina cells with carbohydrate-binding phytoagglutinins: cell surface changes with differentiation, Exp. Cell Res. 70:391CrossRefGoogle Scholar
  25. Laskey, R. A., and Gurdon, J. B. (1973), Induction of polyoma DNA synthesis by injection in frog egg cytoplasm, Europ. J. Biochem. 37:461CrossRefGoogle Scholar
  26. Loeb, L. A., Fansler, B., Williams, R., and Mazia, D. (1969), Sea urchin nuclear DNA polymerase. I. Localization in nuclei during rapid DNA synthesis, Exp. Cell Res. 57:29CrossRefGoogle Scholar
  27. Longo, F. J., and Plunkett, W. (1973), The onset of DNA synthesis and its relation to morphogenetic events of the pronuclei in activated eggs of the sea urchin Arbaciapunctulata, Develop. Biol. 30:56PubMedCrossRefGoogle Scholar
  28. Mazia, D. J. (1949), The distribution of deoxyribonuclease in the developing embryo (Arbacia punctulata), J. Cell. Comp. Physiol. 34:17CrossRefGoogle Scholar
  29. Monroy, A., Ortolani, G., O’Dell, D. S. and Millonig, G. (1973), Binding of Concanavalin A to the surface of unfertilized and fertilized Ascidian eggs,Nature (Lond.) 242:409CrossRefGoogle Scholar
  30. Moore, E. C, and Hurlbert, R. B. (1966), Regulation of mammalian deoxyribonucleotide biosynthesis by nucleotides as activators and inhibitors, J. Biol. Chem. 241:4802PubMedGoogle Scholar
  31. Moscona, A. A. (1971), Embryonic and neoplastic cell surfaces: availability of receptors for Concanavalin A and wheat germ agglutinin, Science 171:905PubMedCrossRefGoogle Scholar
  32. Nagano, H. D., and Mano, Y. (1968), Thymidine kinase, thymidylate kinase, and 32-P1 -(14C) thymidine incorporation into DNA during early embryogenesis for the sea urchin, Biochim. Biophys. Acta 157:546–541PubMedCrossRefGoogle Scholar
  33. Nemer, M. (1962), Characteristic of the utilization of nucleosides by embryos of Paracen-trotus lividus, J. Biol. Chem. 237:143PubMedGoogle Scholar
  34. Noonan, K. D., Levine, A. J., and Burger, M. M. (1973), Cell cycle-dependent changes in the surface membrane as detected with [3H] Concanavalin A,J. Cell Biol. 58:491.PubMedCentralPubMedCrossRefGoogle Scholar
  35. Noronha, J. M., Sheys, G. H., and Buchanan, J. M. (1972), Induction of a reductive pathway for deoxyribonucleotide synthesis during early embryogenesis of the sea urchin, Proc.Natl. Acad. Sei. USA 69:2006CrossRefGoogle Scholar
  36. O’Dell, D. S., Ortolani, G., and Monroy, A. (1974), Increased binding of radioactive Concanavalin A during maturation of Ascidia eggs, Exp. Cell Res., 83:408PubMedCrossRefGoogle Scholar
  37. Ord, M. G., and Stocken, L. A. (1974), Thymidine uptake by Paracentrotus eggs during the first cell cycle after fertilization, Exp. Cell Res. 83:411.PubMedCrossRefGoogle Scholar
  38. Parisi, E., and De Petrocellis, B. (1972), Properties of a deoxyribonuclease from a nuclear extract of Paracentrotus lividus embryos, Bioch. Biophys. Res. Commun. 49:106.CrossRefGoogle Scholar
  39. Reichard, P. (1968), The Biosynthesis of Deoxyribose, Ciba Lecture, John Wiley, N. Y.Google Scholar
  40. Rossi, M., Geraci, G., and Scarano, E. (1967), Deoxycytidylate aminohydrolase. III. Modifications of the substrate sites caused by allosteric effectors, Biochemistry 6:3640.PubMedCrossRefGoogle Scholar
  41. Scarano, E., and Maggio, R. (1959), The enzymatic deamination of 5′-deoxycytidilic and 5′-methyldeoxycytidilic acid in the developing sea urchin embryo, Exp. Cell Res.18:33PubMedCrossRefGoogle Scholar
  42. Scarano, E., Bonaduce, L., and De Petrocellis, B. (1960a), The enzymatic deamination of 6-aminopyrimidine deoxyribonucleotides. II. Purification and properties of a 6-amino- pyrimidine deoxyribonucleoside 5′-phosphate deaminase from unfertilized eggs of sea urchins,J. Biol. Chem. 235:3556PubMedGoogle Scholar
  43. Scarano, E., Talarico, M., Bonaduce, L., and De Petrocellis, B. (1960b), Enzymatic deamination of 5′-deoxycytidylic acid and of 5-methyl-5′-deoxycytidylic acid in growing and in non-growing tissues, Nature (Lond. ) 186:237.CrossRefGoogle Scholar
  44. Scarano, E., Bonaduce, L., and De Petrocellis, B. (1962), The enzymatic aminohydrolysis of 4-aminopyrimidine deoxyribonucleo tides. III. Purification and properties of 2′-deoxy- ribosyl, 4-aminopyrimidone-2, 5′-phosphate aminohydrolase from monkey liver,J. Biol.Chem. 237:3142Google Scholar
  45. Scarano, E., De Petrocellis, B., and Augusti-Tocco, G. (1964), Studies on the control of enzymes synthesis during the early embryonic development of the sea urchin, Bioch.Biophys. Acta 87:114Google Scholar
  46. Vacquier, V. D., Epel, D., and Douglas, L. A. (1972), Sea urchin eggs release protease activity at fertilization, Nature 237:34PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • B. De Petrocellis
    • 1
  • P. Grippo
    • 1
  • A. Monroy
    • 1
  • E. Parisi
    • 1
  • M. Rossi
    • 1
  1. 1.CNR Laboratory of Molecular EmbryologyArcofelice (Naples)Italy

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