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Comparative Aspects of the Biochemistry of Fertilization: Regulatory Mechanisms of DNA Synthesis

  • B. De Petrocellis
  • P. Grippo
  • A. Monroy
  • E. Parisi
  • M. Rossi
Part of the Basic Life Sciences book series (BLSC, volume 4)

Abstract

The onset of the terminal phase of differentiation of the female germinal cell, the transition from oogonium to oocyte, is marked by a suppression of the ability of the cell to replicate its nuclear DNA and divide. After the developing cell undergoes the last oogonial mitotic division, the chromosomes enter the prophase of the first meiotic division, replicate their DNA, and attain the 4C condition. No further overall replication of the DNA takes place throughout the entire period of oocyte growth and until the egg is fertilized. During this long period of oogenesis, however, the oocyte genome is transcriptionally very active: messenger RNAs are synthesized in large amounts. Nevertheless, most of these direct gene products are not immediately translated but are stored in the cytoplasm of the oocyte in order to be available for protein synthesis during the early postfertilization stages of development, stages during which the very high replicative activity of the DNA virtually precludes transcriptional activity.

Keywords

Thymidine Kinase Ribonucleotide Reductase DNase Activity Blastula Stage Thymidylate Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Tdr

2′-deoxythymidine;

dTMP

2′-deoxythymidine-5′-monophosphate;

dTTP

2′-deoxythymidine-5′-triphosphate;

Adr

2′-deoxyadenosine;

dATP

2′-deoxyadenosine-5′-triphosphate;

dCMP

2′-deoxycytidine-5′-monophosphate;

dCTP

2′-deoxycytidine-5′-triphosphate;

polyd(A-T)

copolymer of deoxyadenosine monophosphate and deoxy thymidine monophosphate;

polyrA-polydT

double-stranded homopolymers of adenosine monophosphate and deoxy thymidine monophosphate;

polyrA-d(pT)9

hybrid of the homopolymer polyrA and oligodeoxythymidine monophos­phate;

polydG-polydC

double-stranded homopolymers of deoxyguanosine mono­phosphate and deoxycytidine monophosphate;

DNAse

deoxyribonuclease.

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Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • B. De Petrocellis
    • 1
  • P. Grippo
    • 1
  • A. Monroy
    • 1
  • E. Parisi
    • 1
  • M. Rossi
    • 1
  1. 1.CNR Laboratory of Molecular EmbryologyArcofelice (Naples)Italy

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