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Molecular Mechanisms in Genetic Transformation

  • Jane K. Setlow
Part of the Basic Life Sciences book series (BLSC, volume 8)

Abstract

Molecular mechanisms of recombination of genetic material are most readily studied in microorganisms; a great deal is known about recombination of transforming DNA, introduced into the cell from the medium, with DNA inside bacteria. At present, it is doubtful that this type of transformation will be of use in improving crop plants. However, a new technique of making recombinant DNA molecules from two or more highly divergent organisms may turn out to have great practical importance for agriculture.

Keywords

Genetic Transformation Strand Displacement Deoxyribonucleic Acid Synthesis Haemophilus Injluenzae Cellulose Column Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beattie, K. L. and Setlow, J. K. (1970). Transformation between Haemophilus influenzae and Haemophilus parainfluenzae. J. Bacteriol. 104: 390–400.PubMedGoogle Scholar
  2. Boling, M. E. (1972). Homology between the deoxyribonucleic acids of Haemophilus influenzae and Haemophilus parainfluenzae. J. Bacteriol. 112: 745–750.PubMedGoogle Scholar
  3. Glover, D. M., White, R. L., Finnegan, D. J. and Hogness, D. S. (1975). Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA. Cell 5: 149–157.PubMedCrossRefGoogle Scholar
  4. Kedes, L. H., Cohn, R. H., Lowry, J. C., Chang, A. C. Y., and Cohen, S. N. (1975). The organization of sea urchin histone genes. Cell 6: 359–369.PubMedCrossRefGoogle Scholar
  5. Kelly, T. J., Jr. and Smith, H. 0. (1970). A restriction enzyme from Haemophilus influenzae. II. Base sequence of the recognition site. J. Mol. Biol. 51: 393–409.PubMedCrossRefGoogle Scholar
  6. LeClerc, J. E. and Setlow, J. K. (1974). Transformation in Haemophilus influenzae. In Mechanisms in Recombination ( Grell, R. F., ed.), pp. 187–207. Plenum Press, New York.CrossRefGoogle Scholar
  7. LeClerc, J. E. and Setlow, J. K. (1975). Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae. J. Bacteriol. 122: 1091–1102.PubMedGoogle Scholar
  8. Masamune, Y. and Richardson, C. C. (1971). Strand displacement during deoxyribonucleic acid synthesis at single strand breaks. J. Biol. Chem. 246: 2692–2701.PubMedGoogle Scholar
  9. Morrow, J. F., Cohen, S. N., Chang, A. C. Y., Boyer, H. W., Goodman, H. M., and Helling, R. B. (1974). Replication and transcription of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. USA 71: 1743–1747.PubMedCrossRefGoogle Scholar
  10. Sedgwick, B. and Setlow, J. K. (1976). Single-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae. J. Bacteriol. 125: 588–594.PubMedGoogle Scholar
  11. Smith, H. O. and Wilcox, K. W. (1970). A restriction enzyme from Haemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51: 379–391.Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Jane K. Setlow
    • 1
  1. 1.Brookhaven National LaboratoryUptonUSA

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