Feedback-Resistant Mutants of Histidine Biosynthesis in Yeast

  • Francine Rasse-Messenguy
  • Gerald R. Fink
Part of the Basic Life Sciences book series (BLSC, volume 2)


Mutants resistant to the histidine analog 1, 2, 4-triazole-3-alanine have been isolated and characterized. All members of one group, TRA1, map in the gene encoding the first enzyme of histidine biosynthesis, have a feedback-resistant first enzyme, and are dominant in diploids. As a consequence of the altered feedback properties, some TRA1 mutants have an internal histidine pool 12–15 times higher than that of wild type. In addition to their inability to control the size of their histidine pool, these mutants are unable to derepress normally. Some implications of these results for the study of feedback-resistant strains of higher plants are discussed.


Minimal Medium Amino Acid Pool Amino Acid Analog Histidine Biosynthesis Histidine Concentration 
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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • Francine Rasse-Messenguy
    • 1
  • Gerald R. Fink
    • 1
  1. 1.Department of Genetics, Development, and PhysiologyCornell UniversityIthacaUSA

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