Radioelectrocomplexing: A General Radioimmunoassay Procedure for the Detection of Primary Binding of Antigen by Antibody

  • Malcolm J. Simons
  • Albert A. Benedict


Of the numerous in vitro methods available for estimating the concentration of antigen or antibody in biological fluids those based on the determination of the primary interaction between antigen and antibody have certain advantages over methods in which secondary interactions are measured. The chief advantages are that (1) meaningful thermodynamic and stoichiometric characterization of the reaction can be obtained when antibody and hapten are employed, and (2) nanogram amounts of antigen or antibody can be measured relatively simply and rapidly if the antigen is radioactively labeled.


Human Serum Albumin Immune Complex Veronal Buffer Human Serum Albumin Solution Binding Curf 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Malcolm J. Simons
    • 1
  • Albert A. Benedict
    • 2
  1. 1.WHO Immunology Research and Training CentreUniversity of SingaporeSingaporeSingapore
  2. 2.Department of MicrobiologyUniversity of HawaiiHonoluluUSA

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