NMR Studies of Structure and Conformation in Peptides and Proteins

  • K. Wüthrich
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 22)


Investigations of the relations between covalent structure, molecular conformations and functional properties of biological macromolecules play a prominent role in modern biochemistry, molecular biology, and biophysics. While chemical methods have so far served to unravel the covalent structures of a large number of biopolymers [1], and X-ray crystallography in single crystals has set the standards for the description of the three-dimensional conformations in these molecules [2], NMR has become an attractive technique for investigations of the molecular conformations in solution. To a large extent the high sensitivity and high resolution required for biological applications of NMR have become available by recent advances in apparatus design and biochemical methods, i.e. on the one hand the introduction of superconducting magnets and Fourier transform techniques into the modern NMR spectrometers, on the other hand the preparation of isotope-labelled biopolymers, by chemical and biosynthetic techniques. In the present lecture, some basic notions on structure and conformation of one class of biopolymers, i.e. peptides and proteins, will be introduced, and the manifestations in the NMR parameters of different molecular structures and conformations will be discussed and illustrated with some selected examples.


Torsion Angle Polypeptide Chain Random Coil Amino Acid Side Chain Molecular Conformation 
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  1. 1.
    M.O. DAYHOFF ed., “Atlas of Protein Sequence and Structure”, National Biomedical Research Foundation, Silver Spring, Md. (1969)Google Scholar
  2. 2.
    R.E. DICKERSON and I. GEIS, “The Structure and Action of Proteins”, Harper and Row, New York (1969)Google Scholar
  3. 3.
    G.N. RAMACHANDRAN and V. SASISEKHARAN, Adv.Protein Chem. 23, 283 (1968)CrossRefGoogle Scholar
  4. 4.
    F.A. BOVEY, “Nuclear Magnetic Resonance Spectroscopy”, Academic Press, New York (1969)Google Scholar
  5. 5.
    G.C.K. ROBERTS and O. JARDETZKY, Adv.Protein Chem. 24, 447 (1970)CrossRefGoogle Scholar
  6. 6.
    M. SCHEINBLATT, J.Amer.Chem.Soc. 88, 2845 (1966)CrossRefGoogle Scholar
  7. 7.
    C.C. McDONALD and W.D. PHILLIPS, J.Amer.Chem.Soc. 91, 1513 (1969)CrossRefGoogle Scholar
  8. 8.
    Ch. GRATHWOHL and K. WUTHRICH, J.Magn.Res. 13, 217 (1974)CrossRefGoogle Scholar
  9. 9.
    A. MASSON, Ph.D.Thesis: “Konformationsstudien an Calcitonin M und am Trypsin-Inhibitor BPTI”, ETH Zürich (1974)Google Scholar
  10. 10.
    F.A. BOVEY, A.I. BREWQTER, D.J. PATEL, A.E. TONELLI and D.A. TORCHIA, Acc.Chem.Res. 5, 193 (1972)CrossRefGoogle Scholar
  11. 11.
    R. SCHWYZER, Ch. GRATHWOHL, J.P. MERALDI, A. TUN-KYI, R. VOGEL and K. WUTHRICH, Helv.Chim.Acta 55, 2545 (1972)CrossRefGoogle Scholar
  12. 12.
    V.F. BYSTROV, V.T. IVANOV, S.L. PORTNOVA, T.A. BALASHOVA and Y.A. OVCHINNIKOV, Tetrahedron 29, 873 (1973)CrossRefGoogle Scholar
  13. 13.
    C.C. McDONALD and W.D. PHILLIPS in “Fine Structure of Proteins and Nucleic Acids” eds. G.D. Fasman and S.N. Timasheff, Dekker, New York (1970)Google Scholar
  14. 14.
    K. WUTHRICH, Chimia 24, 409 (1970)Google Scholar
  15. 15.
    K. WUTHRICH, Experientia 30, 577 (1974)CrossRefGoogle Scholar
  16. 16.
    A. MASSON and K. WUTHRICH, FEBS Lett. 31, 114 (1973)CrossRefGoogle Scholar
  17. 17.
    L.A. LA PLANCHE and M.T. ROGERS, J.Amer.Chem.Soc. 86, 337 (1964)CrossRefGoogle Scholar
  18. 18.
    H.L. MAIA, K.G. ORRELL and H.N. RYDON, Chem.Comm. 1971, 1209Google Scholar
  19. 19.
    C.M. DEBER, F.A. BOVEY, J.P. CARVER and E.R. BLOUT, J.Amer.Chem. Soc. 92, 6191 (1970)CrossRefGoogle Scholar
  20. 20.
    K. WUTHRICH, A. TUN-KYI and R. SCHWYZER, FEBS Lett. 25, 104 (1972)CrossRefGoogle Scholar
  21. 21.
    W.A. THOMAS and M.K. WILLIAMS, Chem.Comm. 1972, 994Google Scholar
  22. 22.
    F.A. BOVEY, in “Chemistry and Biology of Peptides”, ed. J. Meienhofer, 3, Ann Arbor Sci.Publ., Ann Arbor (1972)Google Scholar
  23. 23.
    I.C.P. SMITH, R. DESLAURIERS and R. WALTER, in “Chemistry and Biology of Peptides”, ed. J. Meienhofer, 3, Ann Arbor Sci.Publ., Ann Arbor (1972)Google Scholar
  24. 24.
    K. WÜTHRICH and Ch. GRATHWOHL, FEBS Lett. 43, 337 (1974)CrossRefGoogle Scholar
  25. 25.
    A. HVIDT and S.C. NIELSEN, Adv. Protein Chem. 21, 287 (1966)CrossRefGoogle Scholar
  26. 26.
    A. ALLERHAND, D. DODDRELL, V. GLUSHKO, D.W. COCHRAN, E. WENKERT, P.J. LAWSON and F.R.N. GURD, J.Amer.Chem.Soc. 93, 544 (1971)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • K. Wüthrich
    • 1
  1. 1.Eidgenössische Technische HochschuleZürichSwitzerland

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