NMR Studies of Structure and Conformation in Peptides and Proteins
Investigations of the relations between covalent structure, molecular conformations and functional properties of biological macromolecules play a prominent role in modern biochemistry, molecular biology, and biophysics. While chemical methods have so far served to unravel the covalent structures of a large number of biopolymers , and X-ray crystallography in single crystals has set the standards for the description of the three-dimensional conformations in these molecules , NMR has become an attractive technique for investigations of the molecular conformations in solution. To a large extent the high sensitivity and high resolution required for biological applications of NMR have become available by recent advances in apparatus design and biochemical methods, i.e. on the one hand the introduction of superconducting magnets and Fourier transform techniques into the modern NMR spectrometers, on the other hand the preparation of isotope-labelled biopolymers, by chemical and biosynthetic techniques. In the present lecture, some basic notions on structure and conformation of one class of biopolymers, i.e. peptides and proteins, will be introduced, and the manifestations in the NMR parameters of different molecular structures and conformations will be discussed and illustrated with some selected examples.
KeywordsTorsion Angle Polypeptide Chain Random Coil Amino Acid Side Chain Molecular Conformation
Unable to display preview. Download preview PDF.
- 1.M.O. DAYHOFF ed., “Atlas of Protein Sequence and Structure”, National Biomedical Research Foundation, Silver Spring, Md. (1969)Google Scholar
- 2.R.E. DICKERSON and I. GEIS, “The Structure and Action of Proteins”, Harper and Row, New York (1969)Google Scholar
- 4.F.A. BOVEY, “Nuclear Magnetic Resonance Spectroscopy”, Academic Press, New York (1969)Google Scholar
- 9.A. MASSON, Ph.D.Thesis: “Konformationsstudien an Calcitonin M und am Trypsin-Inhibitor BPTI”, ETH Zürich (1974)Google Scholar
- 13.C.C. McDONALD and W.D. PHILLIPS in “Fine Structure of Proteins and Nucleic Acids” eds. G.D. Fasman and S.N. Timasheff, Dekker, New York (1970)Google Scholar
- 14.K. WUTHRICH, Chimia 24, 409 (1970)Google Scholar
- 18.H.L. MAIA, K.G. ORRELL and H.N. RYDON, Chem.Comm. 1971, 1209Google Scholar
- 21.W.A. THOMAS and M.K. WILLIAMS, Chem.Comm. 1972, 994Google Scholar
- 22.F.A. BOVEY, in “Chemistry and Biology of Peptides”, ed. J. Meienhofer, 3, Ann Arbor Sci.Publ., Ann Arbor (1972)Google Scholar
- 23.I.C.P. SMITH, R. DESLAURIERS and R. WALTER, in “Chemistry and Biology of Peptides”, ed. J. Meienhofer, 3, Ann Arbor Sci.Publ., Ann Arbor (1972)Google Scholar