Isolation and Composition of Human Plasma Apolipoproteins
The biochemist’s attempts to understand the structure and function of the human plasma lipoproteins have expanded greatly in recent years due to their possible role in the development of atherosclerosis. Five of the major apolipoproteins have been sequenced and our view of the structural organization of lipoproteins has seen considerable refinement. All of these achievements depend, in one way or another, upon the investigators skill in isolating both intact lipoproteins and apolipoproteins in a highly purified and homogeneous state. For this reason, we present in this chapter a description of some of the tried and proven methods for the purification of the lipoproteins and their component apolipoproteins and lipids. This presentation will not include the purification of abberant lipoproteins; the less frequently used or exotic techniques will be mentioned only to provide a reference for those who may be interested in the more specialized methods. Three reviews of lipoproteins provide ample source material on those areas of lipoprotein structure and function which are outside the scope of this volume (1–3).
KeywordsHigh Density Lipoprotein Cholesteryl Ester Trace Trace Trace Trace Trace Sodium Decyl Sulfate
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