Isolation and Composition of Human Plasma Apolipoproteins

  • Henry J. Pownall
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 15)


The biochemist’s attempts to understand the structure and function of the human plasma lipoproteins have expanded greatly in recent years due to their possible role in the development of atherosclerosis. Five of the major apolipoproteins have been sequenced and our view of the structural organization of lipoproteins has seen considerable refinement. All of these achievements depend, in one way or another, upon the investigators skill in isolating both intact lipoproteins and apolipoproteins in a highly purified and homogeneous state. For this reason, we present in this chapter a description of some of the tried and proven methods for the purification of the lipoproteins and their component apolipoproteins and lipids. This presentation will not include the purification of abberant lipoproteins; the less frequently used or exotic techniques will be mentioned only to provide a reference for those who may be interested in the more specialized methods. Three reviews of lipoproteins provide ample source material on those areas of lipoprotein structure and function which are outside the scope of this volume (1–3).


High Density Lipoprotein Cholesteryl Ester Trace Trace Trace Trace Trace Sodium Decyl Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R.L. Jackson, J.D. Morrisett and A.M. Gotto Jr., Physiological Reviews 56 259 (1976)PubMedGoogle Scholar
  2. 2.
    A.M. Scanu, C. Edelstein and P. Keim in The Plasma Proteins F.W. Putnam, Ed. Academic Press New York (1975) p. 317Google Scholar
  3. 3.
    J.D. Morrisett, R.L. Jackson and A.M. Gotto Jr., Ann. Rev. Biochem. 44 183 (1975)PubMedCrossRefGoogle Scholar
  4. 4.
    V.P. Skipski,in Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism. G.J. Nelson Ed. Wiley New York (1972) p. 471Google Scholar
  5. 5.
    S.J. Friedberg and J.A. Reynolds, J. Biol. Chem. 251 4005 (1976)PubMedGoogle Scholar
  6. 6.
    H.B. Brewer Jr., S.E. Lux, R. Ronan and K.M. John, Proc. Nat. Acad. Sci. USA, 69 1304 (1972)PubMedCrossRefGoogle Scholar
  7. 7.
    H.N. Baker, A.M. Gotto Jr. and R.L. Jackson, Proc. Nat. Acad. Sci. USA, 71 3631 (1974)PubMedCrossRefGoogle Scholar
  8. 8.
    M.O. Dayhoff, Atlas of Protein Sequence and Structure 5 Nat. Biomed. Res. Found. Washington, D.C. (1972)Google Scholar
  9. 9.
    J.D. Morrisett, H.J. Pownall, R.L. Jackson, R. Segura, A.M. Gotto Jr. and O.D. Taunton in: Chemistry and Biochemistry of Polyunsaturated Fatty Acids (W.H. Kunau and R.T. Holman, eds. ) 1976Google Scholar
  10. 10.
    R.S. Shulman, P.N. Herbert, K. Wehrly and D.S. Fredrickson, J. Biol. Chem. 250 12 (1975)Google Scholar
  11. 11.
    R.L. Jackson, J.T. Sparrow, H.N. Baker, J.D. Morrisett, O.D. Taunton and A.M. Gotto Jr., J. Biol. Chem. 249 5308 (1974)PubMedGoogle Scholar
  12. 12.
    R.L. Jackson, H.N. Baker, E.B. Gilliam and A.M. Gotto Jr., (1977) Proc. Nat. Acad. Sci. USA, in pressGoogle Scholar
  13. 13.
    H.B. Brewer, R. Shulman, P. Herbert, R. Ronan and K. Wehrly, J. Biol. Chem. 249 4975 (1974)PubMedGoogle Scholar
  14. 14.
    A.M. Scanu, J. Lipid Res. 7 285 (1966)Google Scholar
  15. 15.
    L.L. Rudel, J.A. Lee, M.D. Morris and J.M. Felts, Biochem. J. 137 89 (1974)Google Scholar
  16. 16.
    G.R. Bartlett, J. Biol. Chem. 234 466 (1959)PubMedGoogle Scholar
  17. 17.
    J.A. Reynolds and R.H. Simon, J. Biol. Chem. 249 3937 (1974)PubMedGoogle Scholar
  18. 18.
    A. Scanu, J. Toth, C. Edelstein, S. Koga and E. Stiller, Biochemistry 8 3309 (1969)PubMedCrossRefGoogle Scholar
  19. 19.
    W.L. Stone and J.A. Reynolds, J. Biol. Chem. 250 3584 (1975)PubMedGoogle Scholar
  20. 20.
    B. Shore and V. Shore, Biochemistry 8 4510 (1969)PubMedCrossRefGoogle Scholar
  21. 21.
    A.M. Scanu and C. Edelstein, Anal. Biochem. 44 176 (1971)CrossRefGoogle Scholar
  22. 22.
    W.V. Brown, T.I. Levy and D.S. Fredrickson, J. Biol. Chem. 244 5687 (1969)PubMedGoogle Scholar
  23. 23.
    J.J. Albers and A.M. Scanu, Biochim. Biophys. Acta 236 29 (1971)PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Henry J. Pownall
    • 1
  1. 1.Baylor College of Medicine and the Methodist HospitalHoustonUSA

Personalised recommendations