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Phospholipase A2 Interaction with Various Phospholipids. A Model for the Lipoprotein Molecule?

  • G. H. de Haas
  • A. J. Slotboom
  • H. M. Verheij
  • J. C. Vidal
  • E. H. J. M. Jansen
  • H. Baartmans
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 15)

Abstract

This NATO-workshop is devoted to the structure and function of the lipoprotein molecule. Important building stones of these complexes are the so-called“apoproteins” and phospholipids. Considerable progress has been made in the isolation and elucidation of the structure of proteins and phospholipids. One of the major problems is how the lipids and proteins interact with each other on a molecular level. Usually reconstitution experiments with pure apoproteins and different types of phospholipids are monitored by calorimetric and spectroscopic techniques such as fluorescence,ultraviolet difference spectroscopy or NMR. However, no sensitive and easy bioassay is available to control the structure of the reconstituted lipoprotein. In addition, the tendency of several apoproteins to aggregate in solution, complicates the interaction study. Therefore comparison might be useful with a modelsystem in which a small water-soluble protein molecule interacts with lipid aggregates, especially if the interaction can be followed by a change in enzyme activity. In this respect lipolytic enzymes are attractive: they are usually small, rather stable proteins, which can easily be obtained in a pure state, and, as we will see, the specific interaction of these proteins with certain lipid-water aggregates is often accompanied by an exponential increase in enzyme activity.

Keywords

Critical Micellar Concentration Lipolytic Enzyme Micelle Complex Pancreatic Phospholipase Lipid Micelle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • G. H. de Haas
    • 1
  • A. J. Slotboom
    • 1
  • H. M. Verheij
    • 1
  • J. C. Vidal
    • 1
  • E. H. J. M. Jansen
    • 1
  • H. Baartmans
    • 1
  1. 1.Laboratory of BiochemistryState University of Utrecht · Transitorium 3, University Centre “De Uithof”UtrechtThe Netherlands

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