Assay of Lipoprotein Lipase in Postheparin Plasma after Suppression of the Hepatic Triglyceride Lipase with Sodium Dodecyl Sulfate

  • Marietta L. Baginsky
  • Flora Wu
  • W. Virgil Brown
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 15)


Lipoprotein lipase (LPL), released by heparin into the circulating blood, is thought to originate from the capillary endothelium of a variety of tissues, including muscle and adipose tissue (1). Much evidence suggests that this enzyme is of primary importance in the degradation and removal of triglyceride from plasma chylomicrons and very low density lipoproteins (2–4). Assay of LPL in postheparin plasma has been complicated by the presence of a second triglyceride lipase which is of hepatic origin (5–7). The function of this hepatic triglyceride lipase (H-TGL) in triglyceride clearance has not been elucidated, though it is possible that it plays a role in the removal of partially degradated lipoproteins following LPL action.


Sodium Dodecyl Sulfate Lipoprotein Lipase Protamine Sulfate Sodium Dodecyl Sulfate Solution Triglyceride Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Robinson, D.S. and J.E. French. 1960. Heparin, the clearing factor lipase, and fat transport. Pharmacol. Rev. 12: 241–263.Google Scholar
  2. 2.
    Scow, R.O., J. Blanchette-Mackie and L.C. Smith. 1976. Role of capillary endothelium in the clearance of chylomicrons. A model for lipid transport from blood by lateral diffusion in cell membranes. Circulation Res. 39: 149–162.Google Scholar
  3. 3.
    Kopiang, I.P., A. Bensadoun, and M.W. Yang. 1976. Effect of an anti-lipoprotein lipase serum on plasma triglyceride removal. J. Lipid Res. 17: 498–505.Google Scholar
  4. 4.
    Jackson, R.L., J.D. Morrisett, and A.M. Gotto, Jr. 1976. Lipoprotein structure and metabolism. Physiological Rev. 56: 259–299.Google Scholar
  5. 5.
    La Rosa, J.C., R.I. Levy, H.G. Windmueller, and D.S. Fredrickson. 1972. Comparison of the triglyceride lipase of liver, adipose tissue, and post-heparin plasma. J. Lipid Res. 13: 356–363.Google Scholar
  6. 6.
    Greten, H., A.D. Sniderman, J.G. Chandler, D. Steinberg, and W.V. Brown. 1974. Evidence for the hepatic origin of canine postheparin plasma triglyceride lipase. FEBS Letters 42: 157–160.CrossRefGoogle Scholar
  7. 7.
    Ehnholm, C., W. Shaw, H. Greten, W. Lengfelder and W.V. Brown. 1974. Separation and characterization of two tri-glyceride lipase activities from human postheparin plasma. In: Atherosclerosis III. Proceedings 3rd. International Symposium. G. Schetler and A. Winzer, Eds. Springer-Verlag, Berlin -Heidelberg -New York, NY, 557–560.CrossRefGoogle Scholar
  8. 8.
    Ehnholm, C., W. Shaw, H. Greten and W.V. Brown. 1975. Purification from human plasma of a heparin-released lipase with activity against triglyceride and phospholipids. J. Biol. Chem. 250: 6756–6761.Google Scholar
  9. 9.
    Brown, W.V., W. Shaw, M. Baginsky, J. Boberg and J. Augustin. 1976. In: Lipoprotein Metabolism, H. Greten, Ed. Springer-Verlag, Berlin–Heidelberg–New York, NY, 2–6.Google Scholar
  10. 10.
    Greten, H., B. Walter and W.V. Brown. 1972. Purification of a human post-heparin plasma triglyceride lipase. FEBS Letters 27: 306–310.CrossRefGoogle Scholar
  11. 11.
    Nilsson-Ehle, P., T. Egelrud, P. Belfrage, T. Olivecrona and B. Borgström. 1973. Positional specificity of purified milk lipoprotein lipase. J. Biol. Chem. 248: 6734–6737.Google Scholar
  12. 12.
    Baginsky, M.L. and W.V. Brown. 1977. Differential characteristics of purified hepatic triglyceride lipase and lipoprotein lipase from human postheparin plasma. J. Lipid Res. 18: XX.Google Scholar
  13. 13.
    Korn, E.D. 1955. Clearing factor, a heparin-activated lipoprotein lipase. I. Isolation and characterization of the enzyme from normal rat heart. J. Biol. Chem. 215: 1–14.Google Scholar
  14. 14.
    Hamilton, R.L. 1965. Post-heparin plasma lipase from the hepatic circulation. Diss. Abstr. 26: 24.Google Scholar
  15. 15.
    Krauss, R.M., H.G. Windmueller, R.I. Levy and D.S. Fredrickson. 1973. Selective measurement of two different triglyceride lipase activities in rat postheparin plasma. J. Lipid Res. 14: 286–295.PubMedGoogle Scholar
  16. 16.
    Boberg, J., J. Augustin, M.L. Baginsky, P. Tejada, and W.V. Brown. 1974. Quantitative determination of hepatic and lipoprotein lipase activities from human post-heparin plasma. Circulation 50: Suppl. III, 21.Google Scholar
  17. 17.
    Huttunen, J.K., C. Ehnholm, P.K. Kinnunen and E.A. Nikkilä. 1975. An immunochemical method for the selective measuremeni of two triglyceride lipases in human postheparin plasma. Clin. Chim. Acta 63: 335–347.Google Scholar
  18. 18.
    Chen, R.F. 1967. Removal of fatty acids from serum albumin by charcoal treatment. J. Biol. Chem 242: 173–181.Google Scholar
  19. 19.
    Huttenen, H.K., J. Ellingboe, R.C. Pittman, and D. Steinberg 1970. Partial purification and characterization of hormone-sensitive lipase from rat adipose tissue. Biochim. Biophys. Acta 218: 333–346.Google Scholar
  20. 20.
    Reynolds, J.A. and C. Tanford. 1970. The gross conformatio: of protein-sodium dodecyl sulfate complexes. J. Biol. Chem. 245: 5161–5165.Google Scholar
  21. 21.
    Krauss, R.M., R.I. Levy, and D.S. Fredrickson. 1974. Select ive measurement of two lipase activities in postheparin plasma from normal subjects and patients with hyperlipoproteinemia. J. Clin. Invest. 54: 1107–1124.Google Scholar
  22. 22.
    Greten, H., R. DeGrella, G. Klose, W. Rascher, J.L. deGennes, and E. Gjone. 1976. Measurement of two plasma triglyceride lipases by an immunochemical method: Studies in patients with hypertriglyceridemia. J. Lipid Res. 17: 203–210.Google Scholar
  23. 23.
    Brown, W.V., M.L. Baginsky, and C. Ehnholm. (In press). Primary Type I and Type V hyperlipoproteinemia. In: Hyperlipidemia: Diagnosis and Therapy. Rifkind, B. and R.I. Levy; (Eds.) Grune and Stratton, New York, NY.Google Scholar
  24. 24.
    Steinhart, J. and J.A. Reynolds. 1969. Multiple equilibria in proteins. Academic Press, New York, NY; 234–302.Google Scholar
  25. 25.
    Augustin, J., H. Freeze, J. Boberg, and W.V. Brown. 1976. Human postheparin plasma lipolytic activities. In: Lipoprotein Metabolism. H. Greten (Ed). Springer-Verlag, Berlin–Heidelberg–New York, NY: 7–12.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Marietta L. Baginsky
    • 1
  • Flora Wu
    • 1
  • W. Virgil Brown
    • 1
  1. 1.Department of Medicine, School of MedicineUniversity of CaliforniaLa JollaUSA

Personalised recommendations