Vapor-Phase Methods for Quantitative Evaluation of Prostaglandins and Related Compounds in Biological Samples

  • S. Nicosia
  • G. Galli
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 13)


Since their discovery, many procedures have been developed for prostaglandin (PG) analysis, based on different principles: biological assay, radioimmunoassay (which will be discussed in other sections of this book), gas chromatographic and mass spectrometric methods.


High Performance Liquid Chroma Prostaglandin Endoperoxide Eicosatetraenoic Acid Silicic Acid Chromatography Mass Fragmentography 
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  1. 1.
    Jouvenaz, G.H., Nugteren, D.H., Beerthuis, R.K. and Van Dorp, D.A. (1970) A sensitive method for the determination of prostaglandins by gas chromatography with electron-capture detection. Biochim. Biophys. Acta, 202, 231–234.PubMedGoogle Scholar
  2. 2.
    Jouvenaz, G.H., Nugteren, D.H. and Van Dorp, D.A. (1973). Gas chromatographic determination of nanogram amounts of prostaglandins E and F. Prostaglandins, 3, 175–187.PubMedCrossRefGoogle Scholar
  3. 3.
    Guidotti, A., Cheney, D.L., Trabucchi, M., Doteuchi, M., Wang, C. and Hawkins, R.A. (1974) Focussed Microwave Radiation: a Technique to Minimize Post-Mortem Changes of Cyclic Nucleotides, DOPA and Choline and to Preserve Brain Morphology, Neuropharmacol., 13, 1115–1122.CrossRefGoogle Scholar
  4. 4.
    Bosisio, E., Galli, C., Galli, G., Nicosia, S., Spa-Gnuolo, C. and Tosi, L. (1976) Correlation between release of free arachidonic acid and prostaglandin formation in brain cortex and cerebellum. Prostaglandins, 11, 773–781.PubMedCrossRefGoogle Scholar
  5. 5.
    Samuelsson, B., Granstrom, E., Gréen, K., Hamberg, M. and Hammarstrom, S. (1975) Prostaglandins, Ann. Rev. Biochem., 44, 669–695.PubMedCrossRefGoogle Scholar
  6. 6.
    Kelly, R.W. (1973) Method for the Measurement of Prostaglandin F in Biological Fluids by Gas Chromato-graphy-Mass Spectrometry. Anal. Chem., 45, 2079–2082.PubMedCrossRefGoogle Scholar
  7. 7.
    Gréen, K., Granstrom, E., Samuelsson, B. and Axen, U. (1973) Methods for Quantitative Analysis of PGF , PGE , 9a, 11a-Dihydroxy-15-Keto-Prost-5-Enoic Acid and 9a, 11a,15-Trihydroxy-Prost-5-Enoic Acid from Body Fluids Using Deuterated Carriers and Gas Chromatography-Mass Spectrometry. Anal. Biochem., 54, 434–453.PubMedCrossRefGoogle Scholar
  8. 8.
    Hamberg, M. (1973) Quantitative Studies on Prostaglandin Synthesis in Man. II. Determination of the Major Urinary Metabolite of Prostaglandins F and F. Anal. Biochem., 55, 368–378.PubMedCrossRefGoogle Scholar
  9. 9.
    Sweetman, B.J., Watson, J.T., Carr, K., Oates, J.A. and Frolich, J.C. (1973) Quantitative vapor-phase analysis of prostaglandin F in female human urine. Prostaglandins, 3, 385–387.PubMedCrossRefGoogle Scholar
  10. 10.
    Hamberg, M., Svensson, J. and Samuelsson, B. (1974) Prostaglandin Endoperoxides. A New Concept Concerning the Mode of Action and Release of Prostaglandins, Proc. Nat. Acad. Sci. USA, 71, 3824–3828.PubMedCrossRefGoogle Scholar
  11. 11.
    Nicosia, S. and Galli, G. (1975) A mass fragmentogra-phic method for the quantitative evaluation of brain prostaglandin biosynthesis. Prostaglandins, 9, 397–403.PubMedCrossRefGoogle Scholar
  12. 12.
    Carr, K., Sweetman, B.J. and Frolich, J.C. (1976) High performance liquid chromatography of prostaglandins: biological applications. Prostaglandins, 11, 3–14.PubMedCrossRefGoogle Scholar
  13. 13.
    Hubbard, W.C. and Watson, J.T. (1976) Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry. Prostaglandins, 12, 21–35.PubMedCrossRefGoogle Scholar
  14. 14.
    Samuelsson, B., Hamberg, M. and Sweeley, C.C. (1970). Quantitative Gas Chromatography of Prostaglandin E, at the Nanogram Level: Use of Deuterated Carrier and Multiple-Ion Analyzer. Anal. Biochem., 38, 301–304.PubMedCrossRefGoogle Scholar
  15. 15.
    Baczynskyj, L., Duchamp, D.J., Zieserl, J.F., Jr. and Axen, U. (1973) Computerized Quantitation of Drugs by Gas Chromatography-Mass Spectrometry. Anal. Chem., 45, 479–482.PubMedCrossRefGoogle Scholar
  16. 16.
    Axen, U., Baczynskyj, L., Duchamp, D.J., Kirton, K.T. and Zieserl, J.F., Jr. (1973) Differentiation between endogenous and exogenous (administered) prostaglandins in biological fluids. In: Advances in the Biosciences, vol. 9, Bergström, S. Ed., Pergamon Press., pp. 109–116.Google Scholar
  17. 17.
    Axen, U., Gréen, K., Horlin, D. and Samuelsson, B. (1971) Mass spectrometric determination of picomole amounts of prostaglandins E2 and F using synthetic deuterium labeled carriers. Biochem. Biophys. Res. Comm., 45, 519–PubMedCrossRefGoogle Scholar
  18. 18.
    Thompson, C.J., Los, M. and Horton, E.W. (1970). The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry. Life Sci., 9, 983–988.CrossRefGoogle Scholar
  19. 19.
    Pace-Asciak, C. and Wolfe, L.S. (1971) N-Butylboronate derivatives of the F prostaglandins. Resolution of prostaglandins of the E and F series by gas-liquid chromatography. J. Chromatogr., 56, 129–133.PubMedCrossRefGoogle Scholar
  20. 20.
    Gréen, K. (1969) Gas chromatography-mass spectrometry of O-methyloxime derivatives of prostaglandins. Chem. Phys. Lipids, 3 254–272.PubMedCrossRefGoogle Scholar
  21. 21.
    Vane, F. and Horning, M.G. (1969) Separation and characterization of the prostaglandins by gas chromatography and mass spectrometry. Anal. Letters, 2 357–371.CrossRefGoogle Scholar
  22. 22.
    Hamberg, M. (1968) Metabolism of prostaglandins in rat liver mitochondria. Europ. J. Biochem., 6, 135–146.PubMedCrossRefGoogle Scholar
  23. 23.
    Sweetman, B.J., Frolich, J.C. and Watson, J.T. (1973) Quantitative determination of prostaglandins A, B and E in the sub-nanogram range. Prostaglandins, 3, 75–87.PubMedCrossRefGoogle Scholar
  24. 24.
    Unpublished observations from this laboratory.Google Scholar
  25. 25.
    Nicosia, S. and Galli, G. (1974) A rapid gas chromato-graphic-mass spectrometric method for prostaglandin analysis at picomole levels. Anal. Biochem., 61, 192–199.PubMedCrossRefGoogle Scholar
  26. 26.
    Hamberg, M. and Samuelsson, B. (1974) Prostaglandin endoperoxide. Novel transformations of arachidonic acid in human platelets. Proc. Nat. Acad. Sci. USA, 71, 3400–3404.PubMedCrossRefGoogle Scholar
  27. 27.
    Levitt, M.J., Josimovich, J.B. and Broskin K.D. (1972) Analysis of prostaglandins by electron-capture gas chromatography. I. Thermal decomposition of hepta-fluorobutyrate methyl esters. Prostaglandins, 1, 121–131.PubMedCrossRefGoogle Scholar
  28. 28.
    Middleditch, B.S. and Desiderio, D.M. (1972) Gas chromatography of prostaglandin heptafluorobutyrate methyl esters. Prostaglandins, 2, 195–198.PubMedCrossRefGoogle Scholar
  29. 29.
    Wickramasinghe, A.J.F. and Shawt R.S. (1974) An electron-capture gas-1iquid-chromatographic method for the determination of prostaglandin F in biological fluids Biochem. J., 141, 179–187.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • S. Nicosia
    • 1
  • G. Galli
    • 1
  1. 1.Institute of Pharmacology and Pharmacognosy, Laboratory of Applied BiochemistryUniversity of MilanMilanItaly

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