Studies on the Molecular Properties of Nerve Growth Factor and its Cellular Biosynthesis and Secretion
NGF was first discovered as a soluble diffusible factor present in 2 mouse sarcomas. This factor has never been isolated and purified, and thus its chemical properties are unknown.
Male adult mouse submandibular glands contain large amounts of NGF, whose covalent structure is known, and male glands contain much more than female glands.
Little, if any, NGF is present in the glands of newborn mice--a perplexing observation since the factor is believed to play a central role in the embryonic development of the autonomic and sensory nervous systems.
Removal of mouse submandibular glands appears to have no obvious deleterious effect upon the animal—and NGF has not been detected in the salivary glands of other mammals.
Treatment of newborn mice with antibody to NGF results in selective destruction of the sympathetic nervous system without other obvious pathologic changes.
Small amounts of a nerve growth-promoting activity have been found in experimentally-induced granulation tissue, serum, and many peripheral tissues—for reasons unknown.
KeywordsNerve Growth Factor Immunoreactive Material Female Gland Mouse Submandibular Gland Nerve Growth Factor Concentration
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- 2.ARNASON, B. G. W., OGER, J., PANTAZIS, N. J. and YOUNG, M., J. Clin. Invest. 53 (1974) 2a.Google Scholar
- 7.BRADSHAW, R. A. and YOUNG, M. , Biochem. Pharm. (1976) in press.Google Scholar
- 10.EARLE, W. R. , J. Nat. Cancer Inst. 4 (1943) 165.Google Scholar
- 14.GOLDSTEIN, M. N., BRODEUR, G. M. and ROSS, D., Anat. Rec. 175 (1973) 330.Google Scholar
- 20.LEVI-MONTALCINI, R. and ANGELETTI, P. U., Regional Neurochemistry (Kety, S. S. and Elkes, J. eds.) Pergamon Press, Oxford (1961) 362.Google Scholar
- 27.PANTAZIS, N. J., OGER, J., ARNASON, B. G. W. and YOUNG, M., Fed. Proc. 33 (1974) 1343.Google Scholar
- 30.SAIDE, J. D., MURPHY, R. A., CANFIELD, R. E., SKINNER, J., ROBINSON, D. R., ARNASON, B. G. W. and YOUNG, M., J. Cell Biol. 67 (1975) 376a.Google Scholar
- 34.STEWARD, H. L. , SNELL, K. C, DUNHAM, L. J. and SCHLYEN, S. M., Transplantable and Transmissible Tumors of Animals (Armed Forces Institute of Pathology, Sec. 12, F40 Washington, D. C.)(1956) 243, 324.Google Scholar