Concanavalin a Induced Changes of Membrane-Bound Lysolecithin Acyltransferase of Thymocytes
One of the major problems of immunobiology today is the elucidation of the mechanism by which an antigen is able to specifically trigger lymphocytes into mitosis, thereby inducing the clonal expansion necessary for a competent immune response. However, the number of thymus dependent T-cells and bone-marrow derived B-cells which exhibit this sensitivity are so few in number that adequate biological studies are limited (see ref. 17). With the discovery of Nowell (20) that phytohemagglutinin (PHA), a protein extract of the red kidney bean, non-specifically induces blastogenesis in human peripheral blood lymphocytes, the door was opened for model studies of the antigen-lymphocyte interaction. The validity of such model studies is based on the similarity of the response of lymphocytes to PHA and the jack-bean mitogen concanavalin A (Con A), and that of antigen mediated blastogenesis. Some of these analogous reactions are the increase in RNA (16) and DNA synthesis (24), the induction of lymphocyte cytotoxicity in T-cells (21), and immuno-globulin synthesis in B-cells, when the latter are stimulated by Con A cross-linked to a solid surface (1). In addition, the excretion of such biologically active substances as macrophage inhibiting factor (MIF), lymphotoxin, chemotactic and mitogenic factors have been observed for both the antigen and lectin stimulation of lymphocytes (see ref. 15).
KeywordsPyruvate Carboxylase Human Peripheral Blood Lymphocyte Acyl Transferase Acyltransferase Activity Hill Plot
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