Transcription of SV40 in Lytically Infected and Transformed Cells

  • Joe Sambrook
  • Arlene Jackson
  • Walter Keller
  • Brad Ozanne
  • Phillip A. Sharp
  • Bill Sugden
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)


As long as we take reasonable care, we can grow stocks of Simian Virus that are remarkably homogeneous. Each infectious particle contains a single molecule of DNA weighing 3.4 x 106 daltons—enough to code for perhaps 5–8 polypeptides of average size. The manner in which this virus-coded information is expressed is highly cell-dependent. For example, after infection with SV40, monkey cells undergo a productive or lytic response, during which there is an ordered appearance of virus-specific functions, with some virus products present at all times and others detectable only in the late stages of the viral growth cycle. The infection progresses through a well-defined series of episodes, which culminate in cell death and the concomitant liberation of a new crop of virus particles. The events are multiplicity-independent in that they occur in the same order, albeit at different rates, in cells infected either with one infectious particle or with a hundred. By contrast, the outcome of exposing mouse cells to SV40 is very different.


Viral Genome Monkey Cell LYTICALLY Infect Endonuclease EcoRI Separate Strand 
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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Joe Sambrook
    • 1
  • Arlene Jackson
    • 1
  • Walter Keller
    • 1
  • Brad Ozanne
    • 1
  • Phillip A. Sharp
    • 1
  • Bill Sugden
    • 1
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA

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