Simian Virus 40 in Synchronized Monkey Cells: Integration of Viral DNA into the Cell Genome and Timing of Replication
Synchronized CV1, cells which were in the G1 period at the time of infection, were able to promote the onset of SV40 DNA replication during the S period immediately following infection. In cells which had passed the end of G1 at the time of infection, viral DNA replication did not start until the S period of the next mitotic cycle. This showed that a cell controlled event, situated at the G1-S interval, conditioned the permissiveness of the host cell to the replication of the viral genome. In addition, initiation of SV40 DNA synthesis required the presence of at least one functional viral gene product, as was shown through the use of the tsA30 mutant of SV40.
Integration of SV40 DNA into the host cell chromosomal DNA was progressive with time after infection and seemed independent of the timing of the host cell cycle.
KeywordsSucrose EDTA Recombination Bromide MgCl2
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