The Repeated Sequences in Serially Passaged SV40 DNA

  • N. Frenkel
  • S. Rozenblatt
  • E. Winocour
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)


Three populations of altered SV40 DNA molecules, independently derived by serial passage of plaque-purified virus at high multiplicity of infection, were found to contain reiterated viral sequences, as determined by reassociation kinetic experiments. In each case, the reiterated sequences corresponded to some of those contained in Hin-fragment s C or D, obtained by digesting plaque-purified virus DNA with Hemophilus influenzae restriction endonuclease. It is suggested that the reiteration of sequences in Hin-fragment C, which is known (Danna and Nathans, 1972; Fareed, Ganon, and Salzman, 1972) to contain the origin of SV40 DNA replication, is responsible for the advantageous replication rate of altered viral genomes during serial passage.

A lack of uniformity was observed in the reassociation rates of different Hin-fragments (cleaved from plaque-purified SV40 DNA) incubated in the presence of increasing concentrations of plaque-purified virus DNA. The possibility that this finding is an indication of sequence duplication in the plaque-purified SV40 genome is discussed.


Viral Sequence Serial Passage Hybridization Reaction Sequence Duplication SV40 Genome 
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  1. Ando, T. 1966. Biochim. Biophys. Acta 114:158–168,PubMedCrossRefGoogle Scholar
  2. Britten, R. J. 1969. Carnegie Inst. Yearbook 67:332–335.Google Scholar
  3. Britten, R. J., and D. E. Kohne. 1968. Science 161:529–540.PubMedCrossRefGoogle Scholar
  4. Brockman, W. W., T. N. H. Lee, and D. Nathans. 1973. Virology 54: 384–397.PubMedCrossRefGoogle Scholar
  5. Danna, K. J., and D. Nathans. 1971. Proc. Nat. Acad. Sei. U.S.A. 68:2913–2917.CrossRefGoogle Scholar
  6. Danna, K. J., and D. Nathans. 1972. Proc. Nat. Acad. Sei. U.S.A. 69:3097–3100.CrossRefGoogle Scholar
  7. Danna, K. J., G. H. Sack, and D. Nathans. 1973. J. Mol. Biol. 78: 363–376.PubMedCrossRefGoogle Scholar
  8. Falk, W. R., and H. C. E. Wang. 1974. Virology 61:140–155.CrossRefGoogle Scholar
  9. Fareed, G. C., J. C. Byrne, and M. A. Martin. 1974. J. Mol. Biol. 87:275–288.PubMedCrossRefGoogle Scholar
  10. Fareed, G. C., C. F. Ganon, and N. P. Salzman. 1972. J. Virol. 10:484–491.PubMedGoogle Scholar
  11. Frenkel, N., S. Lavi, and E. Winocour. 1974. Virology 60:9–20.PubMedCrossRefGoogle Scholar
  12. Hirt, B. 1967. J. Mol. Biol. 26:365–369.PubMedCrossRefGoogle Scholar
  13. Khoury, G., G. C. Fareed, K. Berry, and M. A. Martin. 1974. J. Mol. Biol. 87:289–301.PubMedCrossRefGoogle Scholar
  14. Lavi, S., S. Rozenblatt, M. F. Singer, and E. Winocour. 1972. J. Virol. 12:492–500.Google Scholar
  15. Lavi, S., and E. Winocour. 1972. J. Virol. 9:309–316.PubMedGoogle Scholar
  16. Martin, M. A., L. D. Gelb, G. C. Fareed, and J. B. Milstien. 1973. J. Virol. 12:748–757.PubMedGoogle Scholar
  17. Rozenblatt, S., S. Lavi, M. F. Singer, and E. Winocour. 1972. J. Virol. 12:501–510.Google Scholar
  18. Sutton, W. D. 1971. Biophys. Acta 240:522–531.CrossRefGoogle Scholar
  19. Tai, H. T., C. A. Smith, P. A. Sharp, and J. Vinograd. 1972. J. Virol. 9:317–325.PubMedGoogle Scholar
  20. Wetmur, J. G., and N. Davidson. 1968. J. Mol. Biol. 31:349–370.PubMedCrossRefGoogle Scholar
  21. Winocour, E., N. Frenkel, S. Lavi, M. Osenholts, and S, Rozenblatt. In press. Cold Spring Harbor Symp. Quant. Biol, 39.Google Scholar
  22. Yoshiki, K. 1968a. Virology 34:391–401.CrossRefGoogle Scholar
  23. Yoshiki, K. 1968b. Virology 34:402–409.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • N. Frenkel
    • 1
  • S. Rozenblatt
    • 1
  • E. Winocour
    • 1
  1. 1.Virology SectionWeizmann Institute of ScienceRehovotIsrael

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