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The Repeated Sequences in Serially Passaged SV40 DNA

  • N. Frenkel
  • S. Rozenblatt
  • E. Winocour
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)

Abstract

Three populations of altered SV40 DNA molecules, independently derived by serial passage of plaque-purified virus at high multiplicity of infection, were found to contain reiterated viral sequences, as determined by reassociation kinetic experiments. In each case, the reiterated sequences corresponded to some of those contained in Hin-fragment s C or D, obtained by digesting plaque-purified virus DNA with Hemophilus influenzae restriction endonuclease. It is suggested that the reiteration of sequences in Hin-fragment C, which is known (Danna and Nathans, 1972; Fareed, Ganon, and Salzman, 1972) to contain the origin of SV40 DNA replication, is responsible for the advantageous replication rate of altered viral genomes during serial passage.

A lack of uniformity was observed in the reassociation rates of different Hin-fragments (cleaved from plaque-purified SV40 DNA) incubated in the presence of increasing concentrations of plaque-purified virus DNA. The possibility that this finding is an indication of sequence duplication in the plaque-purified SV40 genome is discussed.

Keywords

Viral Sequence Serial Passage Hybridization Reaction Sequence Duplication SV40 Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • N. Frenkel
    • 1
  • S. Rozenblatt
    • 1
  • E. Winocour
    • 1
  1. 1.Virology SectionWeizmann Institute of ScienceRehovotIsrael

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