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An Analysis of the Structure of the Replicating Fork During Discontinuous Synthesis of SV40 DNA and the Detection of Gap Circle Intermediates

  • Philip J. Laipis
  • A. J. Levine
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)

Abstract

Polyoma and SV40 are small DNA tumor viruses whose well-defined properties have made them ideal subjects for intensive investigation over the last ten years (Black, 1968; Eckhart, 1968; Crawford, 1969; Green, 1970; Sambrook, 1972; Butel, Tevethia and Melnick, 1972). The DNA of these viruses has been extensively characterized by Vinograd and his collaborators (Vinograd and Lebowitz, 1966; Bauer and Vinograd, 1968; Radloff, Bauer and Vinograd, 1967) and by Crawford (Crawford and Black, 1964; Crawford, 1969). They have shown that the genome of SV40 is a single closed-circular molecule of double-stranded DNA containing about 12–15 negative superhelical turns and has a molecular weight of about 3 x 106 daltons. Over the last four years experiments from a number of laboratories working on the mechanism of SV40 DNA replication have yielded a clear outline of the events of viral DNA replication (for a recent review see Levine, 1973). These events may be conveniently divided into four stages: 1) initiation, 2) polynucleotide chain propagation, 3) segregation of the two newly-made daughter molecules, and 4) maturation of the progeny molecules.

Keywords

Replicate Fork Replicative Intermediate African Green Monkey Kidney Cell Polynucleotide Chain Oligonucleotide Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Philip J. Laipis
    • 1
  • A. J. Levine
    • 1
  1. 1.Department of Biochemical SciencesPrinceton UniversityPrincetonUSA

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