An Analysis of the Structure of the Replicating Fork During Discontinuous Synthesis of SV40 DNA and the Detection of Gap Circle Intermediates

  • Philip J. Laipis
  • A. J. Levine
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)


Polyoma and SV40 are small DNA tumor viruses whose well-defined properties have made them ideal subjects for intensive investigation over the last ten years (Black, 1968; Eckhart, 1968; Crawford, 1969; Green, 1970; Sambrook, 1972; Butel, Tevethia and Melnick, 1972). The DNA of these viruses has been extensively characterized by Vinograd and his collaborators (Vinograd and Lebowitz, 1966; Bauer and Vinograd, 1968; Radloff, Bauer and Vinograd, 1967) and by Crawford (Crawford and Black, 1964; Crawford, 1969). They have shown that the genome of SV40 is a single closed-circular molecule of double-stranded DNA containing about 12–15 negative superhelical turns and has a molecular weight of about 3 x 106 daltons. Over the last four years experiments from a number of laboratories working on the mechanism of SV40 DNA replication have yielded a clear outline of the events of viral DNA replication (for a recent review see Levine, 1973). These events may be conveniently divided into four stages: 1) initiation, 2) polynucleotide chain propagation, 3) segregation of the two newly-made daughter molecules, and 4) maturation of the progeny molecules.


Replicate Fork Replicative Intermediate African Green Monkey Kidney Cell Polynucleotide Chain Oligonucleotide Fragment 
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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Philip J. Laipis
    • 1
  • A. J. Levine
    • 1
  1. 1.Department of Biochemical SciencesPrinceton UniversityPrincetonUSA

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