Stability of Avian Oncornavirus Precursor Protein in a Line of Rsv-transformed Hamster Cells
Antibody to disrupted avian myeloblastosis virus was used to precipitate rous sarcoma virus-specific proteins from extracts of RSV-transformed hamster cells. These cells and heterologous transformed cells in general are known not to produce virus particles. When analyzed by dodecyl sulfate gel electrophoresis, the immune precipitates were shown to contain a polypeptide with exactly the same mobility as the AMV polypeptide that has been demonstrated previously to be a precursor to AMV structural proteins in infected chick cells. Tryptic fingerprints indicated that the hamster cell-RSV-polypeptide and the AMV-precursor are closely related. Unlike the AMV-precursor in chick cells, however, the hamster cell-RSV-polypeptide is not cleaved proteolytically to yield virion proteins. It is suggested that the block to virus production in mammalian cells transformed by avian oncornaviruses may be due to the inability of such cells to process the viral precursor polypeptide.
KeywordsVirus Production Rous Sarcoma Virus Avian Myeloblastosis Virus Precursor Polypeptide Hamster Cell Line
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