The Provirus of Rous Sarcoma Virus: Synthesis, Integration and Transcription
RNA-directed DNA synthesis by detergent-disrupted virions of Rous sarcoma virus initiates by the covalent attachment of pdA to the 3′-terminal rA of a 4S RNA hydrogen-bonded to the 70S RNA template. This 4S “primer” has structural features of tRNA and can be aminoacylated with methionine. Synthesis and integration of provirus DNA can be monitored in both permissive (duck) and non-permissive (mouse) cells acutely infected with Rous sarcoma virus. Synthesis of provirus is a prerequisite for both viral replication and cellular transformation by the virus. However, neither integration of viral genes into the host chromosome nor transcription from these genes is necessarily followed by transformation.
KeywordsRous Sarcoma Virus Isoleucine Leucine Visna Virus High Molecular Weight Subunit Duck Cell
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