Characterization and Metabolism of Nuclear Viral RNA Synthesized 18 Hours after Infection with Adenovirus 2
Nuclear viral RNA was studied in cultured human cells (KB) productively infected with adenovirus type 2. The nuclear RNA synthesized beginning 18 hours after infection was at least 50% virus specified. Only 10% of the nuclear RNA bound to poly U filters. More than half of newly synthesized nuclear viral RNA sedimented as greater than 28S, whereas cytoplasmic viral mRNA is of the size range 10S–28S. Nuclear RNA greater than 28S bound to poly U filters 5%, and nuclear RNA in the size range 10S–28S bound nearly 20%.
The metabolic stability of various classes of nuclear viral RNA was analyzed using a “cold chase” method. Total nuclear RNA, nuclear RNA sedimenting as greater than 28S, nuclear RNA sedimenting as 18S-28S and the molecules containing poly A from each of these three classes, all exhibited a slow decay with a half life of 3-5 hours.
KeywordsMetabolic Stability Sucrose Density Gradient Centrifugation Chase Period Chase Experiment Polyuridylic Acid
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