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The Mechanism of Replication of Adenovirus Type 5 DNA

  • J. S. Sussenbach
  • P. C. van der Vliet
  • D. J. Ellens
  • J. Vlak
  • H. S. Jansz
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 5)

Abstract

The virions of adenovirus type 5 (Ad5) DNA contain a linear double-stranded DNA molecule with a length of 11 ym corresponding to a molecular weight of 23 million daltons (Eb, Kesteren, and Bruggen, 1969; Green et al., 1967). Pearson and Hanawalt (1971) have shown that by labeling adenovirus-infected HeLa cells with 3H-thymidine for 5 minutes, the major fraction of the label entered into a fast-sedimenting “replication complex.” The label could be chased from this complex into mature adenovirus DNA. The DNA isolated from these complexes showed an increased buoyant density in CsCl as compared to mature adenovirus DNA. This suggested the presence of single-stranded DNA or RNA in the replicative forms of adenovirus DNA.

Keywords

Genome Length Buoyant Density Adenovirus Type Replicative Intermediate Denaturation Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bellett, A. J. D., and H. B. Younghusband. 1972. J. Mol. Biol. 72:691.PubMedCrossRefGoogle Scholar
  2. Doerfler, W., and A. K. Kleinschmidt. 1970. J. Mol. Biol. 50:579.PubMedCrossRefGoogle Scholar
  3. Eb, A. J. van der. 1973. Virology 51:11.PubMedCrossRefGoogle Scholar
  4. Eb., A. J. van der, L. W. van Kesteren, and E. F. J. van Bruggen. 1969. Biochim. Biophys. Acta 182:530.PubMedCrossRefGoogle Scholar
  5. Green, M., M. Pina, R. Kimes, P. C. Wensink, L. A. MacHattie, and C. A. Thomas. 1967. Proc. Nat. Acad. Sei. U.S.A. 57:1302.CrossRefGoogle Scholar
  6. Pearson, G. D., and P. C. Hanawalt. 1971. J. Mol. Biol. 62:65.PubMedCrossRefGoogle Scholar
  7. Sussenbach, J. S., and P. C. van der Vliet. 1972. FEBS Letters 21:7.PubMedCrossRefGoogle Scholar
  8. Sussenbach, J. S., and P. C. van der Vliet. 1973. Virology 54:299.PubMedCrossRefGoogle Scholar
  9. Sussenbach, J. S., P. C. van der Vliet, D. J. Ellens, and H. S. Jansz. 1972. Nature New Biol. 239:47.PubMedGoogle Scholar
  10. Vliet, P. C. van der, and J. S. Sussenbach. 1972. Europe. J. Biochem. 30:584.CrossRefGoogle Scholar
  11. Winnacker, F. L., G. Magnusson, and P. Reichard. 1971. Biochem. Biophys. Res. Comm. 44:952.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • J. S. Sussenbach
    • 1
  • P. C. van der Vliet
    • 1
  • D. J. Ellens
    • 1
  • J. Vlak
    • 1
  • H. S. Jansz
    • 1
  1. 1.Laboratory for Physiological ChemistryState University of UtrechtUtrechtThe Netherlands

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