Ribonucleic Acid Synthesis in Plants

  • Donald Grierson
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


The two large RNA components of the ribosomes of the plant cytoplasm have molecular weights of 1.3 and 0.7 x 106. These molecules are synthesized in the nucleolus as part of a polycistronic precursor which is subsequently methylated and cleaved in a number of processing steps to produce functional rRNA (1). Polyacrylamide gel electrophoresis of pulse-labelled RNA from the nuclei of aseptically grown Phaseolus aureus seedlings shows that the rRNA precursors and processing intermediates have molecular weights of approximately 2.6, 1.45, and 1.0 x 106. The precursors have a similar base composition to rRNA and competitive hybridi-zation experiments show that the large precursor contains sequences for both the 1.3 and 0.7 x 106 molecular weight rRNAs (2). Saturation hybridization experiments between rRNA and nuclear DNA shows that 0.6–0.9% of the DNA codes for rRNA.


Hybridization Experiment Processing Intermediate Acer Pseudoplatanus Large Precursor Diploid Nucleus 
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  1. 1.
    Grierson, D., Rogers, M.E., Sartirana, M.L., and Loening, U.E. (1970) Cold Spring Harbor Symp. Quant. Biol. 35, 589–598.Google Scholar
  2. 2.
    Grierson, D. and Loening, U.E. (1974) Eur. J. Biochem. 44, 501–507.PubMedCrossRefGoogle Scholar
  3. 3.
    Grierson, D. and Smith, H. (1973) Eur. J. Biochem. 36, 280–285.PubMedCrossRefGoogle Scholar
  4. 4.
    Grierson, D. (1974) Eur. J. Biochem. 44, 509–515.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Donald Grierson
    • 1
  1. 1.Department of Physiology and Environmental StudiesUniversity of Nottingham, School of Agriculture Sutton BoningtonLoughborough, LeicsGt. Britain

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