Properties of 5-Bromodeoxyuridine-Resistant Lines of Higher Plant Cells in Liquid Culture
5-Bromodeoxyuridine (BUdR)-resistant cells were obtained from N-methyl-N’-nitro-N-nitrosoguanidine (NTG)-treated soybean protoplasts and cultured in liquid nutrient medium containing BUdR (20 μg/ml)and uridine (100 μg/ml). Addition of uridine to the medium improved growth of the BUdR-resistant cells.
KeywordsLiquid Culture Resistant Cell Thymidine Kinase Regional Laboratory Gradient Analysis
- K. Ohyama, Properties of 5-bromodeoxyuridine-resistant lines of higher plant cells in liquid culture. Expt. Cell Res., 1974, in press.Google Scholar