Properties of 5-Bromodeoxyuridine-Resistant Lines of Higher Plant Cells in Liquid Culture

  • Kanji Ohyama
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


5-Bromodeoxyuridine (BUdR)-resistant cells were obtained from N-methyl-N’-nitro-N-nitrosoguanidine (NTG)-treated soybean protoplasts and cultured in liquid nutrient medium containing BUdR (20 μg/ml)and uridine (100 μg/ml). Addition of uridine to the medium improved growth of the BUdR-resistant cells.


Liquid Culture Resistant Cell Thymidine Kinase Regional Laboratory Gradient Analysis 
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  1. K. Ohyama, Properties of 5-bromodeoxyuridine-resistant lines of higher plant cells in liquid culture. Expt. Cell Res., 1974, in press.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Kanji Ohyama
    • 1
  1. 1.Prairie Regional LaboratoryNational Research Council of CanadaSaskatoonCanada

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