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Properties of 5-Bromodeoxyuridine-Resistant Lines of Higher Plant Cells in Liquid Culture

  • Kanji Ohyama
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)

Abstract

5-Bromodeoxyuridine (BUdR)-resistant cells were obtained from N-methyl-N’-nitro-N-nitrosoguanidine (NTG)-treated soybean protoplasts and cultured in liquid nutrient medium containing BUdR (20 μg/ml)and uridine (100 μg/ml). Addition of uridine to the medium improved growth of the BUdR-resistant cells.

Keywords

Liquid Culture Resistant Cell Thymidine Kinase Regional Laboratory Gradient Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. K. Ohyama, Properties of 5-bromodeoxyuridine-resistant lines of higher plant cells in liquid culture. Expt. Cell Res., 1974, in press.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Kanji Ohyama
    • 1
  1. 1.Prairie Regional LaboratoryNational Research Council of CanadaSaskatoonCanada

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