Uptake of DNA and Bacteriophage into Pollen and Genetic Manipulation

  • Dieter Hess
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


Among higher plants protplasts, tissues cultures and plant embryos are currently being discussed as systems of genetic manipulation (Review: Hess,1974). The first successes have already been attained on tissue cultures (Doy et al.1973 a, b, c; Johnson et al. 1973) and plant embryos (Review: Hess,1972; Ledoux et al.,1974), whereas the first successes on protoplasts are at least being heralded (Review: Chaleff and Carlson,1974). I confess that I too, like so many others, succumbed at first to the fascination of the protoplast system introduced mainly by Cocking (Power et al.,1970; Review: Cocking 1972). However, once one has regenerated some hundreds of petunias from isolated protoplasts(Hess and Potrykus, 1972; Hess et al.,1973; Donn et al., 1973), one knows that protoplasts do not offer only advantages. Regenerating a large number of plants from isolated protoplasts is coupled with a comparatively high expenditure of time and technology. In order to be able to carry on genetic manipulations with a chance of success, however, large numbers are necessary.


Pollen Tube Genetic Manipulation Germination Medium Phage Lambda Petunia Hybrida 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chaleff,R.S., and P.S.Carlson: Ann.Rev.Genetics 8, in press (1974).Google Scholar
  2. Cocking,E.C.: Ann.Rev.Plant Physio1. 23, 29 (1972).CrossRefGoogle Scholar
  3. Donn G., D.Hess und I.Potrykus: Z.Pflanzenphysiol. 69, 423 (1973).Google Scholar
  4. Doy,C.H., P.M.Gresshoff and B.G.Rolfe: Proc.Austral. Biochem.Soc. 5, 3 (1972).Google Scholar
  5. Doy,C.H. P.M.Gresshoff and B.G.Rolfe: in Pollack and Lee (eds.), The Biochemistry of Gene Expression in Higher Organisms, p. 21 (1973 a).Google Scholar
  6. Doy,C.H.,P.M.Gresshoff and R.G.Rolfe:Proc.Nat.Acad. Sci. USA 70, 723 (1973 b).PubMedCrossRefGoogle Scholar
  7. Doy,C.H.,P.M.Gresshoff and R.G.Rolfe: Nature New Biology 244, 90 (1973 c).PubMedGoogle Scholar
  8. Hess,D.: Naturwissenschaften 59, 348 (1972).PubMedCrossRefGoogle Scholar
  9. Hess,D.: Biol.Rdsch. 12, in press (1974).Google Scholar
  10. Hess,D., I.Potrykus, G.Donn and F.Hoffmann: Colloques internationaux C.N.R.S. 212, 343 (1973).Google Scholar
  11. Hess,D., H.Lörz und E.Weissert: Z.Pflanzenphysio1.74, in press (1974).Google Scholar
  12. Hess,D., P.M.Gresshoff, U.Fielitz and D.Gleiss: Z.Pflanzenphysiol. in press (1975).Google Scholar
  13. Hoffmann,F.:Z.Pflanzenphysiol.69, 249 (1973).Google Scholar
  14. Hoffmann,F., and D.Hess: Z.Pflanzenphysio1. 69, 81 (1973).Google Scholar
  15. Johnson,C.B., D.Grierson and H.Smith: Nature New Biology 244, 105 (1973).CrossRefGoogle Scholar
  16. Ledoux,L., R.Huart and M.Jacobs: Nature 249, 17 (1974).PubMedCrossRefGoogle Scholar
  17. Merril,C.R., and H.Stanbro: Z.Pflanzenphysiol. 72, 371 (1974).Google Scholar
  18. Nitsch,C.: C.R.Acad.Sci.Paris,Serie D, 278, 1031 (1974).Google Scholar
  19. Power J.B. S.E.Cummins and E.C.Cocking: Nature 225, 1016 (1970).PubMedCrossRefGoogle Scholar
  20. Rangaswamy,N.S., and K.R.Shivanna: Nature 216, 937 (1967).CrossRefGoogle Scholar
  21. Wagner,G., and D.Hess: Z.Pflanzenphysiol. 69, 262 (1973).Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Dieter Hess
    • 1
  1. 1.University of HohenheimStuttgart-70Germany

Personalised recommendations