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DNA-Hybridization Studies of the Fate of Bacterial DNA in Plants

  • A. Kleinhofs
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)

Abstract

Evidence for the uptake and integration of foreign (bacterial) donor DNA into plants, following application of donor DNA to seeds, has been reported in several systems by Ledoux and his collaborators (12,14). In some experiments, radioactive donor DNA is used, while in others, unlabeled DNA is applied and treated plants are subsequently labeled with 3H-thymidine. In both cases, the evidence for covalent joining of donor high buoyant density DNA and recipient low buoyant density DNA is the occurrence of a peak of radioactivity of intermediate density in treated plants. This intermediate peak upon sonication separates into components of approximately donor and recipient buoyant density. These observations are taken as evidence for joining of foreign DNA sequences. However, buoyant density is not a sufficient criterion to identify as donor bacterial DNA the “heavy” component which separates from intermediate density DNA upon sonication. One needs a criterion which identifies base sequences, not just the gross base composition of the DNA. Thus, DNA hybridization measurements are essential to support the model which Ledoux and his collaborators have proposed. This study was undertaken in order to provide such an analysis of intermediate density DNA.

Keywords

Intermediate Density Buoyant Density Barley Genome Fixed Angle Rotor CsCl Density Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • A. Kleinhofs
    • 1
  1. 1.Department of RadiobiologyCentre d’Etude de l’Energie Nucleaire CEN/SCKMolBelgium

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