Isozymes and a Strategy for Their Utilisation in Plant Genetics II. Isozymes as a Tool in Plant Genetics

  • Michel Jacobs
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


Since 1961, the year where the National Academy of Sciences of the United States of America has sponsored a first symposium on the multiple molecular forms of enzymes, numerous reports have ap­peared which relate isozymes to various topics and this establish­ment of electrophoretic variants of enzymes in various organisms has led to a number of research applications in genetics, biochemistry and physiology. A review of recent advances in plant genetics mainly based on the use of isozymes in the goal of this paper. We shall first describe research areas where isozymes have played and still play a major role.


Alcohol Dehydrogenase Limited Factor Gene Dosage Effect Hybrid Band Datura Stramonium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    Scandalios J.G. and L.G. Espiritu, 1969. Mutant aminopeptidase of Pisum sativum. Molecular and General Genetics, 105: 101–112.PubMedCrossRefGoogle Scholar
  2. (2).
    Jacobs, M. and Schwind, F. 1974. Biochemical genetics of Arabidopsis acid phosphatase polymorphism, tissue expression and genetics of AP1, AP2 and AP3 loci. 3rd Int. Conf. on Isozymes, C.L. Markert, ed. Academic Press, N.Y. ( in press)Google Scholar
  3. (3).
    Chourey P.S. H.H. Smith and N.C. Combatti. 1973. Effects of X irradiations and indoleacetic acid on specific peroxidase isozymes in pith tissue of a Nicotiana amphiploid. Am. J. Bot. 60: 853–857.Google Scholar
  4. (4).
    Longo G.P. and J.G. Scandalios, 1969. Nuclear gene control of mitochondrial malic dehydrogenase in maize. Proc. Nat. Acad. Sci. U.S.A., 62: 104–111.Google Scholar
  5. (5).
    Allard R.W. and A.L. Kahler, 1971, Allozyme polymorphisms in plant populations. Stadler Symposia, 3: 9–24.Google Scholar
  6. (6).
    Gottbier, L.D. 1974. Gene duplication and fixed heterozygosity for alcohol dehydrogenase in Clarkia franciscana. Proc. Nat. Acad. Sci. U.S.A. 71: 1816–18.Google Scholar
  7. (7).
    Sing C.F. and C.J. Brewer, 1969. Isozymes of a polyploid series of wheat.Genetics, 61: 391–398.Google Scholar
  8. (8).
    Johnson B.L., 1972. Seed protein profiles and the origin of the hexaploid wheats. Ann. J. Bot. 59: 952–960.Google Scholar
  9. (9).
    Te Niedenhuis, B. 1971. Estimation of the proportion of inbred seed in Brussels sprouts hybrid seed by acid phosphatase isoenzyme analysis. Euphytica 20: 498–507.Google Scholar
  10. (10).
    Schwartz D., 1971. Genetic control of alcohol dehydrogenase: a competition model for regulation of gene action. Genetics, 67: 41 1425.Google Scholar
  11. (11).
    Efron, Y. 1970. Alcohol dehydrogenase in maize: genetic control of enzyme activity. Science 170: 791–3.Google Scholar
  12. (12).
    Efron Y. 1971. Regulation of the activity of alcohol dehydrogenase isozymes during the development of the maize kernel. Molecular and General Genetics, 111: 97–102.Google Scholar
  13. (13).
    Endo T. 1973. Isozyme loci and a Strategy of Differentiation in Plants. Seiken Zihó, 24 : 89–104.Google Scholar
  14. (14).
    Carlson, P.F. 1972. Locating genetic loci with aneuploids. Mol. Gen. Genetics 114: 273–280.Google Scholar
  15. (15).
    Smith H.H. and M.E. Conklin, 1974. Effects of Gene Dosage on Peroxidase Isozymes in Datura stramonium Trisomies 3rd Int. Conf. on Isozymes, C.H. Markert, e.a. Academic Press. N.Y. (in press).Google Scholar
  16. (16).
    Gillispie, I. and K. Kojima. 1968. The degree of polymorphisms in enzymes involved in energy production compared to that in non specific enzymes in two Drosophila anassanae populations. Proc.Nat. Acad. Sci. U.S.A. 61: 582.Google Scholar
  17. (17).
    Johnson, G.B. 1974. Enzyme polymorphism and metabolism. Science 184: 28–37.Google Scholar
  18. (18).
    Ting I.P. and C.B. Osmond, 1973. Multiple forms of plant phosphoenolpyruvate carboxylase associated with different metabolic pathways. Plant Physiol. 51: 448–453.Google Scholar
  19. (19).
    Schwartz D. and W.J. Laughner, 1969. A molecular bases for heterosis. Science, 166: 626–627.Google Scholar
  20. (20).
    Schwartz D., 1973. Single gene heterosis for alcohol dehydrogenase in maize: the nature of the subunit interaction. Theoretical Applied Genetics, 43: 117–120.Google Scholar
  21. (21).
    Scandalios J.G. E.H. Liu and M.A. Campeau, 1972. The effects of intragenic and intergenic complementation on catalase structure and function in maize: a molecular approach to heterosis. Archives of biochemistry and biophysics, 153: 695–705.Google Scholar
  22. (22).
    Efron Y. 1973. Specific Differences in Maize Alcohol Dehydrogenase: Possible Explanation of Heterosis at the Molecular Level. Nature New Biology, 241: 41–42.Google Scholar
  23. (23).
    Schwartz, D. 1960. Electrophoretic and immunochemical studies with endosperm proteins of maize mutants. Genetics 45: 1419–27.Google Scholar
  24. (24).
    Chourey P.S. 1971. Interallelic complementation at the Sh1 locus in maize. Genetics, 68: 435–442.Google Scholar
  25. (25).
    Bell J. and R. Maclntyre, 1973. Characterization of Acid Phosphatase- 1 Null Activity Mutants of Drosophila melanon_aster, Biochemical Genetics, Vol. 10 no 1: 39–55.Google Scholar
  26. (26).
    Ganapathy P.S. and J.G. Scandalios, 1973. Malate dehydrogenase isozymes in haploid and diploid Datur.a. species: their use as markers in somatic cell genetics. The Journal of Heredity, 64: 186–188.Google Scholar
  27. (27).
    Carlson, P.S. H. Smith and R.D. Dearing. 1972. Parasexual inter-specific plant hybridization. Proc. Nat. Acad. Sci. U.S.A. 69: 2292–4.Google Scholar
  28. (28).
    Jacobs M. 1975. In preparation.Google Scholar
  29. (29).
    Chaleff, R. 1974. Heterogenous association of cells formed in vitro (this symposium).Google Scholar
  30. (30).
    Igave I., M. Nishio and F. Kurasawa, 1973. Occurrence of Acid Phosphatase isozymes Repressible by inorganic Phosphate in Rice Plant Cell Cultures, Agr. Biol. Chem. 37(4): 941-943.Google Scholar
  31. (31).
    Kao, K.N. F. Constabel, H.R. Michayluk and O.L. Gamborg, 1974. Protoplast fusion and growth of intergeneric hybrid cells. Abstract 148, 3rd Int. Cong. Plant Tissue and Cell Culture, Leicester.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Michel Jacobs
    • 1
  1. 1.Laboratorium voor PlantengeneticaVrije Universiteit BrusselSint-Genesius-RodeBelgië

Personalised recommendations