InVitro Selection for Mutants of Higher Plants

  • R. S. Chaleff
  • P. S. Carlson
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


Tobacco plants resistant to wildfire disease have been regenerated from methionine sulfoximine-resistant calluses selected in vitro. The intracellular concentration of free methionine is elevated in two of the three mutant plants. Three rice cell lines which are resistant to the lysine analogue, S-(ß-minoethyl)-cysteine have also been isolated in vitro. Analyses of free and protein-incorporated amino acid composition reveal increased levels of several amino acids in the variant cell lines.


Free Amino Acid Haploid Plant Ethyl Methanesulfonate Automate Amino Acid Analyzer Free Lysine 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • R. S. Chaleff
    • 1
  • P. S. Carlson
    • 1
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA

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